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Date: 2023-05-25 Category: Not Applicable State: Union Government Country: Europe

Commission Implementing Regulation (EU) 2023/1021 of 24 May 2023 renewing the approval of the active substance Bacillus thuringiensis subsp. kurstaki PB 54 in accordance with Regulation (EC) No 1107/2009 of the European Parliament and of the Council, and amending Commission Implementing Regulation (EU) No 540/2011 (Text with EEA relevance)

Issued by European Commission · Directorate-General for Health and Food Safety

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Executive Summary & Key Takeaways

Executive Summary: This Implementing Regulation (EU) 2023/1021 renews the approval of the active substance Bacillus thuringiensis subsp. kurstaki PB 54, in accordance with Regulation (EC) No 1107/2009. It amends Implementing Regulation (EU) No 540/2011. The renewed approval is valid until 30 June 2038, with specific conditions for use. The regulation takes effect on 1 July 2023. Key Points / Main Content: Approval Renewal: * The approval of Bacillus thuringiensis subsp. kurstaki PB 54 is renewed until 30 June 2038. Amendments to Implementing Regulation (EU) No 540/2011: * Entry 195 in Part A of the Annex is deleted. * A new entry (164) for Bacillus thuringiensis subsp. kurstaki PB 54 is added to Part B of the Annex. Conditions of Use: * Member States must consider the review report on Bacillus thuringiensis subsp. kurstaki PB 54, particularly Appendices I and II. * Emphasis must be placed on protecting operators and workers, ensuring adequate personal protective equipment is used. * Producers must maintain strict environmental conditions and quality control during manufacturing to meet microbiological contamination limits. * A minimum of 2 days must be kept between application and harvest for edible crops for fresh consumption, unless residue data shows levels below 105 CFUg at harvest. Additional Data Requirements: * The applicant must submit data regarding the density decline of viable spores on edible plant parts (stone fruits and tomatoes) from application until harvest or until levels are below 105 CFUg, including storage stability data. * The applicant and the Rapporteur Member State must agree on relevant methods and protocols by 14 December 2025. Impact Analysis: Commission: * Impact: Receives additional data from the applicant regarding spore density decline. * Action Required: Review data submissions from applicant Applicant: * Impact: Approval for Bacillus thuringiensis subsp. kurstaki PB 54 is renewed with specific conditions and data requirements. * Action Required: Submit additional data on spore density decline by agreed methods and protocols to the Commission, Member States, and the Authority and agree these methods with the Rapporteur Member State by 14 December 2025. Member States: * Impact: Responsible for assessing applications for plant protection products containing Bacillus thuringiensis subsp. kurstaki PB 54, with particular attention to operator and worker protection. * Action Required: Implement uniform principles, paying attention to operator/worker protection and microbiological contamination limits. Review applications for plant protection products containing Bacillus thuringiensis subsp. kurstaki PB 54. European Food Safety Authority (EFSA): * Impact: Receives additional data from the applicant regarding spore density decline. * Action Required: Review data submissions from applicant.

Key Entities Referenced

Bacillus thuringiensis subsp. kurstaki PB 54: An active substance used in plant protection products, the approval of which is being renewed. Regulation EC No 1107/2009: Regulation of the European Parliament and of the Council concerning the placing of plant protection products on the market. Commission Implementing Regulation EU No 540/2011: Commission Implementing Regulation as regards the list of approved active substances. European Parliament: One of the legislative bodies of the European Union. Council of the European Union: One of the legislative bodies of the European Union. European Food Safety Authority: An agency of the European Union that provides independent scientific advice on food safety. Denmark: The rapporteur Member State for the application for renewal of the approval of the active substance. Netherlands: The co-rapporteur Member State for the application for renewal of the approval of the active substance.
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L 137/16 EN Official Journal of the European Union 25.5.2023 COMMISSION IMPLEMENTING REGULATION (EU) 2023/1021 of 24 May 2023 renewing the approval of the active substance Bacillus thuringiensis subsp. kurstaki PB 54 in accordance with Regulation (EC) No 1107/2009 of the European Parliament and of the Council, and amending Commission Implementing Regulation (EU) No 540/2011 (Text with EEA relevance) THE EUROPEAN COMMISSION, Having regard to the Treaty on the Functioning of the European Union, Having regard to Regulation (EC) No 1107/2009 of the European Parliament and of the Council of 21 October 2009 concerning the placing of plant protection products on the market and repealing Council Directives 79/117/EEC and 91/414/EEC(1), and in particular Article 20(1) thereof, Whereas: (1) Commission Directive 2008/113/EC(2)included a reference to the approval of Bacillus thuringiensis subsp. kurstaki PB 54 as an active substance in Annex I to Council Directive 91/414/EEC(3). (2) According to Article 78(3) of Regulation (EC) No 1107/2009, active substances included in Annex I to Directive 91/414/EEC are deemed to have been approved under Regulation (EC) No 1107/2009 and are listed in Part A of the Annex to Commission Implementing Regulation (EU) No 540/2011(4). (3) The approval of the active substance Bacillus thuringiensis subsp. kurstaki PB 54, as set out in Part A of the Annex to Implementing Regulation (EU) No 540/2011 expires on 15 August 2024. (4) On 30 April 2016, an application for the renewal of the approval of the active substance Bacillus thuringiensis subsp. kurstaki PB 54 was submitted to Denmark, the rapporteur Member State, and the Netherlands, the co-rapporteur Member State, in accordance with Article 1 of Commission Implementing Regulation (EU) No 844/2012(5) and within the time period provided for in that Article. (5) The applicant also submitted the supplementary dossiers required by Article 6 of Implementing Regulation (EU) No 844/2012 to the rapporteur Member State, the co-rapporteur Member State, the Commission and the European Food Safety Authority (‘the Authority’). The application was found to be admissible by the rapporteur Member State. (1) OJ L 309, 24.11.2009, p. 1. (2) Commission Directive 2008/113/EC of 8 December 2008 amending Council Directive 91/414/EEC to include several micro- organisms as active substances (OJ L 330, 9.12.2008, p. 6). (3) Council Directive 91/414/EEC of 15 July 1991 concerning the placing of plant protection products on the market (OJ L 230, 19.8.1991, p. 1). (4) Commission Implementing Regulation (EU) No 540/2011 of 25 May 2011 implementing Regulation (EC) No 1107/2009 of the European Parliament and of the Council as regards the list of approved active substances (OJ L 153, 11.6.2011, p. 1). (5) Commission Implementing Regulation (EU) No 844/2012 of 18 September 2012 setting out the provisions necessary for the implementation of the renewal procedure for active substances, as provided for in Regulation (EC) No 1107/2009 of the European Parliament and of the Council concerning the placing of plant protection products on the market (OJ L 252, 19.9.2012, p. 26). This Regulation was replaced by Commission Implementing Regulation (EU) 2020/1740 (OJ L 392, 23.11.2020, p. 20), however, according to Article 17 of that Regulation, it continues to apply to the procedure for the renewal of the approval of active substances: (1) whose approval period ends before 27 March 2024; (2) for which a Regulation, adopted in accordance with Article 17 of Regulation (EC) No 1107/2009 on or after 27 March 2021, extends the approval period to 27 March 2024 or a later date.25.5.2023 EN Official Journal of the European Union L 137/17 (6) The rapporteur Member State prepared a draft renewal assessment report in consultation with the co-rapporteur Member State and submitted it to the Authority and the Commission on 28 June 2019. In its draft renewal assessment report the rapporteur Member State proposes to renew the approval of Bacillus thuringiensis subsp. kurstaki PB 54. (7) The Authority communicated the draft renewal assessment report to the applicant and to the Member States for comments, launched a public consultation, and forwarded the comments received to the Commission. The Authority also made the supplementary summary dossier available to the public. (8) On 2 March 2021, the Authority communicated to the Commission its conclusion(6), which indicates that Bacillus thuringiensis subsp. kurstaki PB 54 can be expected to meet the approval criteria provided for in Article 4 of Regulation (EC) No 1107/2009. (9) The Commission presented a renewal report regarding Bacillus thuringiensis subsp. kurstaki PB 54 and a draft of this Regulation to the Standing Committee on Plants, Animals, Food and Feed on 14 October 2022and on 25 January 2023, respectively. (10) The Commission invited the applicant to submit its comments on the conclusion of the Authority and, in accordance with Article 14(1), third subparagraph, of Implementing Regulation (EU) No 844/2012, on the renewal report. The applicant submitted its comments, which have been carefully examined and taken into consideration. (11) It has been established with respect to one or more representative uses of at least one plant protection product containing the active substance Bacillus thuringiensis subsp. kurstaki PB 54 that the approval criteria provided for in Article 4 of Regulation (EC) No 1107/2009 are satisfied. (12) It is therefore appropriate to renew the approval of Bacillus thuringiensis subsp. kurstaki PB 54. (13) It is, however, necessary to provide for certain conditions pursuant to Article 14(1), read in conjuction with Article 6, of Regulation (EC) No 1107/2009. It is, in particular, appropriate as a precautionary approach for consumer dietary protection to include a minimum time period between the application of plant protection products containing Bacillus thuringiensis subsp. kurstaki PB 54 and the harvest of edible crops used for fresh consumption, unless residues data show levels of Bacillus thuringiensis subsp. kurstaki PB 54 below 105 CFU/g at harvest. (14) In addition, in order to increase the confidence in the conclusion that Bacillus thuringiensis subsp. kurstaki PB 54 does not affect human health, the applicant should provide further data regarding the density decline of viable spores of Bacillus thuringiensis subsp. kurstaki PB 54 on edible plant parts from the time of application of a plant protection product containing this active substance until the time of harvest or until levels found are below 105 CFU/g. (15) Furthermore, it is appropriate to require that Member States, when assessing applications for authorisations of plant protection products containing Bacillus thuringiensis subsp. kurstaki PB 54, pay particular attention to the protection of operators and workers. (16) Implementing Regulation (EU) No 540/2011 should therefore be amended accordingly. (6) EFSA Journal. DOI: https://doi.org/10.2903/j.efsa.2021.6496. Available online: www.efsa.europa.eu.L 137/18 EN Official Journal of the European Union 25.5.2023 (17) Commission Implementing Regulation (EU) 2023/689(7) extended the approval of Bacillus thuringiensis subsp. kurstaki PB 54 until 15 August 2024in order to allow the renewal process to be completed before the expiry of the approval period of that active substance. However, given that a decision on renewal has been taken ahead of that extended expiry date, this Regulation should apply earlier than that date. (18) The measures provided for in this Regulation are in accordance with the opinion of the Standing Committee on Plants, Animals, Food and Feed, HAS ADOPTED THIS REGULATION: Article 1 Renewal of the approval of the active substance The approval of the active substance Bacillus thuringiensis subsp. kurstaki PB 54, as specified in Annex I, is renewed, subject to the conditions laid down in that Annex. Article 2 Amendments to Implementing Regulation (EU) No 540/2011 The Annex to Implementing Regulation (EU) No 540/2011 is amended in accordance with Annex II to this Regulation. Article 3 Entry into force and application This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union. It shall apply from 1 July 2023. This Regulation shall be binding in its entirety and directly applicable in all Member States. Done at Brussels, 24 May 2023. For the Commission The President Ursula VON DER LEYEN (7) Commission Implementing Regulation (EU) 2023/689 of 20 March 2023 amending Implementing Regulation (EU) No 540/2011 as regards the extension of the approval periods of the active substances Bacillus subtilis (Cohn 1872) strain QST 713, Bacillus thuringiensis subsp. Aizawai strains ABTS-1857 and GC-91, Bacillus thuringiensis subsp. Israeliensis (serotype H-14) strain AM65-52, Bacillus thuringiensis subsp. Kurstaki strains ABTS 351, PB 54, SA 11, SA12 and EG 2348, Beauveria bassiana strains ATCC 74040 and GHA, clodinafop, Cydia pomonella Granulovirus (CpGV), cyprodinil, dichlorprop-P, fenpyroximate, fosetyl, malathion, mepanipyrim, metconazole, metrafenone, pirimicarb, pyridaben, pyrimethanil, rimsulfuron, spinosad, Trichoderma asperellum (formerly T. harzianum) strains ICC012, T25 and TV1, Trichoderma atroviride (formerly T. harzianum) strain T11, Trichoderma gamsii (formerly T. viride) strain ICC080, Trichoderma harzianum strains T-22 and ITEM 908, triclopyr, trinexapac, triticonazole and ziram (OJ L 91, 29.3.2023, p. 1).ANNEX I Common Name, Identification IUPAC Expiration of Purity(1) Date of approval Specific provisions Numbers Name approval Bacillus thuringiensis subsp. n.a. No relevant 1 July 2023 30 June 2038 The implementation of the uniform principles, as provided for in Article 29(6) of kurstaki PB 54 impurities Regulation (EC) No 1107/2009, requires that the conclusions of the review report on Bacillus thuringiensis subsp. kurstaki PB 54 and in particular Appendices I and II thereto, are taken into account. In this overall assessment, the Member States shall pay particular attention to: — the protection of operators and workers, taking into account that micro-organisms are per se considered as potential sensitisers, ensuring that adequate personal protec­ tive equipment is included as a condition of use; — the assurance by the producer of strict maintenance of environmental conditions and quality control analysis during the manufacturing process, in order to ensure the fulfilment of the limits on microbiological contamination as referred to in the Working Document SANCO/12116/2012(2). Conditions of use shall include the following risk mitigation measures: — a minimum time period of 2 days shall be kept between the application of plant pro­ tection products containing Bacillus thuringiensis subsp. kurstaki PB 54 and the har­ vest of edible crops used for fresh consumption, unless available measured or esti­ mated residues data show levels of Bacillus thuringiensis subsp. kurstaki PB 54 below 105 CFU/g at harvest. The applicant shall submit to the Commission, the Member States and the Authority additional information as regards: — data in at least one representative edible crop (i.e. on stone fruits and tomatoes) regarding the density decline of viable spores of Bacillus thuringiensis subsp. kurstaki PB 54 on edible plant parts from the time of application of a plant protection pro­ duct containing this active substance until the time of harvest or until levels found are below 105 CFU/g, including storage stability data of the micro-organisms between the sampling and the spore counting analysis. The relevant methods and protocols to be employed shall be agreed between the applicant and the Rapporteur Member State by 14 December 2025. (1) Further details on the identity and the specification of the active substance are provided in the renewal report. (2) pesticides_ppp_app-proc_guide_phys-chem-ana_microbial-contaminant-limits.pdf (europa.eu). 25.5.2023 EN Official Journal of the European Union L 137/19ANNEX II The Annex to Implementing Regulation (EU) No 540/2011 is amended as follows: (1) in part A, entry 195 is deleted, (2) in Part B, the following entry is added: Common Name, IUPAC Expiration of No. Purity(1) Date of approval Specific provisions Identification Numbers Name approval ‘164 Bacillus thuringiensis n.a. No relevant 1 July 2023 30 June 2038 The implementation of the uniform principles, as provided for in Article 29(6) of subsp. kurstaki PB 54 impurities Regulation (EC) No 1107/2009, requires that the conclusions of the review report on Bacillus thuringiensis subsp. kurstaki PB 54 and in particular Appendices I and II thereto, are taken into account. In this overall assessment, the Member States shall pay particular attention to: — the protection of operators and workers, taking into account that micro-organisms are per se considered as potential sensitisers, ensuring that adequate personal protec­ tive equipment is included as a condition of use; — the assurance by the producer of strict maintenance of environmental conditions and quality control analysis during the manufacturing process, in order to ensure the ful­ filment of the limits on microbiological contamination as referred to in the Working Document SANCO/12116/2012(2). Conditions of use shall include the following risk mitigation measures: — a minimum time period of 2 days shall be kept between the application of plant pro­ tection products containing Bacillus thuringiensis subsp. kurstaki PB 54 and the harvest of edible crops used for fresh consumption, unless available measured or estimated residues data show levels of Bacillus thuringiensis subsp. kurstaki PB 54 below 105 CFU/g as recommended by EFSA. The applicant shall submit to the Commission, the Member States and the Authority additional information as regards: — data in at least one representative edible crop (i.e. on stone fruits and tomatoes) regarding the density decline of viable spores of Bacillus thuringiensis subsp. kurstaki PB 54 on edible plant parts from the time of application of a plant protection product containing this active substance until the time of harvest or until levels found are below 105 CFU/g, including storage stability data of the micro-organisms between the sampling and the spore counting analysis. The relevant methods and protocols to be employed shall be agreed between the applicant and the Rapporteur Member State by 14 December 2025. (1) Further details on the identity and the specification of the active substance are provided in the renewal report. (2) pesticides_ppp_app-proc_guide_phys-chem-ana_microbial-contaminant-limits.pdf (europa.eu).’ L 137/20 EN Official Journal of the European Union 25.5.2023

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