This notification, issued by the Bureau of Indian Standards (BIS), Department of Consumer Affairs on July 30, 2025, announces the establishment of new Indian Standards according to Subrule 1 of Rule 15 of the Bureau of Indian Standards Rules, 2018.
Specifically, the notification establishes the following standards, effective July 28, 2025:
1. **IS 5835: 2025:** *Determination of Vitamin D in Fortified Vegetable Oils by Ultra-High Performance Liquid Chromatography and Tandem Mass Spectrometry (UHPLC-MS/MS) Method of Test.* This standard supersedes IS 5835: 1970 (*Method for Estimation of Vitamin D in Foodstuffs First Revision*), which will be withdrawn on January 28, 2026.
2. **IS 7234: 2025:** *Determination of Vitamin B9 (Folates) in Foodstuffs by Ultra-High Performance Liquid Chromatography and Tandem Mass Spectrometry (UHPLC-MS/MS) Method of Test.* This standard supersedes IS 7234: 1974 (*Method for Estimation of Folic Acid in Foodstuffs*), which will be withdrawn on January 28, 2026.
3. **IS 19308: 2025:** *Determination of Vitamin B12 in Food Using Liquid Chromatography and Tandem Mass Spectrometry (LC-MS/MS) Method of Test.* There are no existing standards being superseded by this new standard.
The notification was issued by Rachna Sehgal, Scientist G and DDG MSC, and carries reference number HQPUB01312020PUBBIS 1300.
Key Entities Referenced
Bureau of Indian Standards: An organization that establishes Indian standards as mentioned in the notification.
Department of Consumer Affairs: A government department to which the Bureau of Indian Standards is related, as indicated in the notification.
New Delhi: The city where the notification was issued.
Bureau of Indian Standards Rules, 2018: A set of rules governing the Bureau of Indian Standards. Subrule 1 of Rule 15 is referenced in the notification.
IS 5835 : 2025: A newly established Indian Standard for Determination of Vitamin D in Fortified Vegetable Oils.
IS 7234 : 2025: A newly established Indian Standard for Determination of Vitamin B9 Folates in Foodstuffs.
IS 19308 : 2025: A newly established Indian Standard for Determination of Vitamin B12 in Food.
Mayapuri, New Delhi: Location of the Government of India Press, Ring Road.
रजिस्ट्री स.ं डी.एल.- 33004/99 REGD. No. D. L.-33004/99
सी.जी.-डी.एल.-अ.-06082025-265228
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CG-DL-E-06082025-265228
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असाधारण
EXTRAORDINARY
भाग III—खण् ड 4
PART III—Section 4
प्राजधकार स ेप्रकाजित
PUBLISHED BY AUTHORITY
स.ं 537] नई दिल्ली, मगं लिार, अगस्ट्त 5, 2025/श्रािण 14, 1947
No. 537] NEW DELHI, TUESDAY, AUGUST 5, 2025/SHRAVANA 14, 1947
भारतीय मानक ब्य रू ो
(उपभोक्त ा मामल ेजिभाग)
अजधसचू ना
नई दिल्ली 30 िलु ाई, 2025
सिं भ:भ HQ-PUB013/1/2020-PUB-BIS (1300).—भारतीय मानक ब्यूरो जनयम, 2018 के जनयम 15 के
उपजनयम (1) के अनुसरण म ें भारतीय मानक ब्यूरो एतिद्वारा अजधसूजचत करता ह ै की जिन भारतीय मानकों के जििरण
इसमें संलग्न अनूसूची के जद्वतीय स्ट्तंभ म ें दिये गय े हैं, तीसरे स्ट्तभं में इंजगत जतजि को स्ट्िाजपत हो गये हैं। चौिे स्ट्तंभ में दिय े
गय े मानकों के जििरण, यदि कोई हो तो ि े भी साि-साि लाग ू रहगें े िब तक ि े पााँचि ें स्ट्तंभ में इंजगत जतजि को िापस जलए
िाने ह।ैं
अनसु चू ी
क्रम स्ट्िाजपत भारतीय मानकों की सख्ं या, प्रजतस्ट्िापन भारतीय मानकों, यदि िापस जलए िापस होने की
सं. जतजि जतजि
िर्भ तिा िीर्भक िाने ह,ैं की संख्या, िर् भ तिा
िीर्भक
(1) (2) (3) (4) (5)
1 आई एस 5835 : 2025 28 िुलाई आई एस 5835 – 1970 28 िनिरी
2025 2026
अल्रा-हाई परफॉरमेंस जलदिड खाद्य पिािों में जिटाजमन डी के
5215 GI/2025 (1)2 THE GAZETTE OF INDIA : EXTRAORDINARY [PART III—SEC.4]
क्रोमैटोग्राफी और टेंडम मास आंकलन की पद्धजत
स्ट्पेक्रोमेरी (यूएचपीएलसी-
एमएस/एमएस) द्वारा फोर्टभफाइड
िनस्ट्पजत तेलों म ेंजिटाजमन डी ज्ञात
करना — परीक्षण पद्धजत
(पहला पुनरीक्षण)
2 आई एस 7234 : 2025 28 िुलाई आई एस 7234 – 1974
2025
अल्रा-हाई परफॉरमेंस जलदिड खाद्य पिािों में फोजलक एजसड के 28 िनिरी
क्रोमैटोग्राफी और टेंडम मास आंकलन की पद्धजत 2026
स्ट्पेक्रोमेरी (यूएचपीएलसी-
एमएस/एमएस) द्वारा खाद्य पिािों म ें
जिटाजमन बी9 (फोलेट्स) ज्ञात करना
— परीक्षण पद्धजत
(पहला पुनरीक्षण)
3 आई एस 19308 : 2025 28 िुलाई लाग ू नहीं लाग ू नहीं
2025
जलदिड क्रोमैटोग्राफी और टेंडम मास
स्ट्पेक्रोमेरी (एलसी-एमएस/एमएस)
द्वारा खाद्य पिािों म ेंजिटाजमन बी12
ज्ञात करना — परीक्षण पद्धजत
रचना सहगल, िैज्ञाजनक िी एिं उप महाजनिेिक (एम एस सी)
[जिज्ञापन-III/4/असा./270/2025-26]
BUREAU OF INDIAN STANDARDS
(DEPARTMENT OF CONSUMER AFFAIRS)
NOTIFICATION
New Delhi, the 30th July, 2025
Ref: HQ-PUB013/1/2020-PUB-BIS (1300).—In pursuance of Sub-rule (1) of Rule (15) of the Bureau of
Indian Standards Rules, 2018, the Bureau of Indian Standards hereby notifies that Indian standards, particulars of
which are given in the second column of the schedule hereto annexed have been established on the date indicated
against it in third column. The particulars of the standards, if any which are given in the fourth column shall also
remain in force concurrently till they are withdrawn on the date indicated against them in the fifth column.
SCHEDULE
Sl No., Year & Title of the Indian Date of No. , Year & Title of the Date of
No. Standards Established Establishment Indian Standards to be Withdrawal
Withdrawn, if any
(1) (2) (3) (4) (5)
IS 5835 : 2025
1 Determination of Vitamin D in Fortified 28 July 2025 IS 5835 – 1970 28 January
Vegetable Oils by Ultra-High Method for Estimation of 2026
Performance Liquid Chromatography Vitamin D in Foodstuffs
and Tandem Mass Spectrometry
(UHPLC-MS/MS) — Method of Test
(First Revision)[भाग III—खण् ड 4] भारत का रािपत्र : असाधारण 3
2 IS 7234 : 2025 28 July 2025 IS 7234 – 1974 28 January
Determination of Vitamin B9 (Folates) Method for Estimation of 2026
in Foodstuffs by Ultra-High Folic Acid in Foodstuffs
Performance Liquid Chromatography
and Tandem Mass Spectrometry
(UHPLC-MS/MS) — Method of Test
(First Revision)
3 IS 19308 : 2025 28 July 2025 NA NA
Determination of Vitamin B12 in Food
Using Liquid Chromatography and
Tandem Mass Spectrometry (LC-
MS/MS) — Method of Test
RACHNA SEHGAL, Scientist G & DDG (MSC)
[ADVT.-III/4/Exty./270/2025-26]
Uploaded by Dte. of Printing at Government of India Press, Ring Road, Mayapuri, New Delhi-110064
and Published by the Controller of Publications, Delhi-110054.