Guidelines for Recognition of Environmental Laboratories under the Environment (Protection) Act

LABORATORY ANALYTICAL TECHNIQUES SERIES LATS/9/2024-2025 (Revised & Updated Version, December 2024) GUIDELINES FOR RECOGNITION OF ENVIRONMENTAL LABORATORIES UNDER THE ENVIRONMENT

(PROTECTION) ACT, 1986 CENTRAL POLLUTION CONTROL BOARD (MINISTRY OF ENVIRONMENT & FORESTS) `Parivesh Bhawan’, East Arjun Nagar Delhi -110 032 E-mail: cpcb@alpha.nic.in; Website: www.cpcb.nic.in MoEF&CC Website: www.envfor.nic.in December 2024CONTENTS Page No.

1.0 INTRODUCTION

1.1 Environmental Laboratory 01

1.2 Legal Provisions for Recognition of Environmental Laboratory 01

1.2.1 The Environment (protection) Act, 1986 01-02

1.2.2 Functions of Environmental Labs as specified under Rule 9 of EPA, 1986 02

1.3 Objectives for Recognition of the Laboratory under the Environment (Protection) 02-03 Act, 1986

1.4 Delegation of powers for Recognition of Environmental Laboratories under the 03 Environment (Protection) Act, 1986 to Central Pollution Control Board by MoEF&CC

1.5 Benefits of Recognition under EPA, 1986 to the Laboratory 03-04

2.0 REQUISITE GUIDELINES FOR APPLICATION FOR RECOGNITION OF ENVIRONMENTAL LABORATORIES UNDER THE E(P) ACT, 1986

2.1 Recognition under the Environment (Protection) Act, 1986 05

2.2 Who can apply for Recognition - Category of the Laboratory 05

2.3 Application Fees for Recognition 05-06

2.4 How to Apply for Recognition 06-07

2.5 Duration of Recognition 07

2.6 Expiry of Recognition 07

2.7 Renewal of Recognition 07

2.8 Submission of application for Renewal of Recognition 07

2.9 Pre-requisite Requirements for application 07-08

2.10 Details of All the Mandatory Requirements of EPA Recognition 08

2.10.1 Laboratory Legal Entity/Certificate of Incorporation /Registration / 08 Authorization

2.10.2 Laboratory Site /Location in designated authorized area 08

2.10.3 Mandatory Accreditation & Certification 08-09

2.10.4 Submission of Detailed Scope of ISO 17025 Accreditation 09

2.10.5 Laboratory Total Work Area 09

2.10.6 Man-Power-Details 09-10

2.10.7 Laboratory Organization Structure 10-11

2.10.8 Work Allotment Details 112.10.9 Training Details of Man power 11

2.10.10 Code of Ethics & Integrity of Analytical Reports 11

2.10.11 GST/PAN Registration 11

2.10.12 Geo tagging Details 11

2.10.13 Laboratory Photographs 11-12

2.10.14 Laboratory Video Clipping 12

2.10.15 Environmental Parameters analyzed and details of their Analytical 12 Method, Range of analysis, MDL etc.

2.10.16 Use of Validated Analytical Methods 12-13

2.10.17 List of Reference Material/Certified Reference Material 13

2.10.18 Technical Infrastructure and Equipment 13

2.10.19 Calibration status of important instruments / equipment with accepted 13 validity

2.10.20 Maintenance of Laboratory Instruments/Equipment 13

2.10.21 Participation in Inter-Laboratory Analytical Quality Control (IQC) / 13 Proficiency Testing (PT)

2.10.22 Compulsorily Participation in Inter-Laboratory Analytical Quality 13-14 Control (AQC) conducted by CPCB

2.10.23 CPCB AQC Participation Fees 14

2.10.24 Government Analyst 14

2.10.25 Nomination for proposed Govt. Analysts 15

2.10.26 Submission of Bio Data by respective nominated Govt. Analyst 15

2.10.27 Terms & Conditions for Recognition of Government Analyst 15

2.10.28 Substitution of Govt. Analyst during the period of recognition 15

2.10.29 Authorized Signatory of Analysis Reports issued by recognized lab 15

2.10.30 Acceptance of Terms & Conditions for Recognition of Laboratory 15 under the Environment (Protection) Act 1986 by Head of Laboratory

2.10.31 Bio-Metric Attendance System 15-16

2.10.32 Laboratory Waste generation (Liquid waste, Solid waste, Spent 16 Solvents, Bio-hazardous waste) collection and Disposal practices

2.10.33 Other Requirements for Best Laboratory Practices 16-17

2.11 Documentation Checklist for fulfilment of requirement of Recognition-Documents 17-18 Checklist for completeness of Application (New/Renewal) for Recognition of the Laboratory under The Environment (Protection) Act, 1986

2.12 Submission of Duly Completed Application 18

2.13 Amendments/changes with application proforma and evaluation criteria 18

2.14 Scrutiny / processing of Application 182.15 Communication of shortcomings in the infrastructure facilities of the laboratory 18

2.16 Rejection of Application (if found incomplete or non - fulfilment of pre-requisite 18 criteria).

2.17 Meeting all the requirements as per the Checklist on Web-portal 18-19

2.18 Grant of Recognition and Gazette Notification 19

2.19 Expert Committee 19

2.20 Procedure for Processing and Evaluation of Environmental Laboratory for 19 Consideration of Recognition under the Environment (Protection) Act 1986

2.21 Time limit for Disposal of application 19

2.22 Use of Phrase “Recognized under the Environment (Protection) Act, 1986” 20

2.23 Obligation of the Laboratory during the period of Recognition 20

2.24 Desktop Audit, Surveillance, and Surprise Inspection of recognized laboratories 20

2.25 Inspection Team 20

2.26 Actions to be taken against non-compliance 20-22

2.27 Penalty for contravention of the provisions of the Act and rules, orders and directions 22

2.28 Show Cause Notice & Revoking of Recognition 22 Annexures

ANNEXUREI: Undertaking To Follow Code Of Ethics

ANNEXURE II: Details of Laboratory Personnel at the Laboratory as per Organization chart

ANNEXURE III: List of Mandatory Analytical Parameters For Recognition

ANNEXURE IV: List of all the other parameters may be applied for Recognition

ANNEXUREV: Analytical Methods

ANNEXUREVI: List of Sampling and sampling processing Instruments/Equipment

ANNEXURE VII: Analytical Instruments at Environmental laboratories

ANNEXUREVIII: Laboratory Safety / Protection Equipment

ANNEXUREIX: CPCB Inter-laboratory AQC Participation Fees

ANNEXUREX: Terms & Conditions For Recognition Of Laboratories Under The Environment

(Protection) Act, 1986

ANNEXUREXI: Terms And Conditions Acceptance By Proposed Government Analysts Under The Environment (Protection) Act, 1986

ANNEXUREXII: Bio-Data Proforma For Consideration Of Recognition As Govt. Analyst Under The Environment (Protection) Act, 1986

ANNEXURE XIII: Draft Gazette Notification ADDENDUM Addendum I Copy of Gazette Notification No S.O 145 (E), dated 21st February 1991 for Delegation of powers for recognition of Environmental Laboratories under the Environment

(Protection) Act, 1986 to Central Pollution Control Board by MoEF&CC Addendum II Copy of Gazette Notification No S.O.2340 (E), dated 16th June, 2021 for Delegation of powers for recognition of Environmental Laboratories under the Environment

(Protection) Act, 1986 to Central Pollution Control Board by MoEF&CC Addendum III Amendment Notification S.O. 2409 ( E ), dated 19th June 2024 Addendum IV Glossary of Terms - Laboratory Quality Assurance (QA) and Quality Control (QC) system Sampling and analysis charges applicable at CPCB laboratories for Environmental Samples (Please refer Gazette Notification No. 163, dated 23rd Feb 2022).CHAPTER 1.0 INTRODUCTION

1.1 Environmental Laboratory Environmental laboratory plays predominant role in assessing the status of environment comprising both abiotic (soil, water and air) and biotic (flora, fauna and human being) components. Environmental laboratory is a building where processing and testing of samples collected from the environmental media (air, emissions, water, waste water, soil, sludge, sediments biota etc.) is taking place. The environmental laboratory is also responsible for monitoring of industrial solid waste, municipal solid waste, hazardous waste, biomedical waste etc. that affect the environment, humans and wildlife.

The environmental laboratories are the essential cornerstones of any effective pollution control programme, that provide qualitative as well as quantitative data for appropriate decision making. For generating this valuable data with a desired accuracy and to quantify concentration of the constituents present in the samples, the laboratory should have the standard facilities, trained manpower and capabilities to achieve the above goals. The qualifications and experience of laboratory technicians / personnel, play a significant role in generating standardized protocols, proper techno- scientific sampling of environmental matrices and their accurate and quality analysis to produce scientifically reliable and legally defensible data.

“For successful implementation of environmental protection programme, it is essential to identify and quantify the pollution sources, pollutants, conduct baseline survey, lay down standards and build-up monitoring systems”. A competent laboratory is required to meet out these requirements with all necessary instruments, equipment, expertise, capabilities etc.

Present Guideline intends to provide a comprehensive manual describing about the complete requirements that has to be fulfilled by the environmental laboratories seeking for Recognition under The Environment (Protection) Act, 1986. This guideline also explains the complete procedure of recognition right from the registration to the publication of gazette notification.

1.2 Legal Provisions of Recognition of Environmental Laboratory The need for laboratories in implementation of the various Pollution Control Acts laid down for the protection of the environment is essential under the following sections of Act.

1.2.1 The Environment (Protection) Act, 1986 Under Section 12 (1) The Central Government may, by notification in the Official Gazette- a) establish one or more laboratories, b) Recognize one or more laboratories or institutes as environmental laboratories to carry out the functions entrusted to an environmental laboratory under this Act.

Under section 12 (2) The Central Government may, by notification in the Official Gazette, make rules specifying- 1a) the functions of the environmental laboratory b) the procedure for the submission to the said laboratory of samples of water, air, soil or other substance for analysis or tests, the form of the laboratory report there on and the fees payable for such report;

c) such other matters as may be necessary or expedient to enable that laboratory to carry out its functions.

Under Section (13) The Central Government may by notification in the official Gazette, appoint or recognize such persons as it thinks fit and having the prescribed qualification to be Government Analysts for the purpose of analysis of samples of water, air, soil or other substances sent for analysis to any environmental laboratory established or recognized under sub-section (1 ) of section 12 of The Environment ( Protection) Act, 1986.

1.2.2 Functions of Environmental Labs as specified under Rule 9 of The Environment

(Protection) Rules, 1986 i. To evolve standardized methods for sampling and analysis of various types of environmental pollutants. ii. To analyze samples sent by the Central Government or the officers empowered under sub-section (1) of section 11 of E(P)A, 1986 iii. To carry out such investigations as may be directed by the Central Government to lay down standards for the quality of environment and discharge of environmental pollutants, to monitor and to enforce the standards laid down.

iv. To send periodical reports regarding its activities to the Central Government. v. To carry out such other functions as may be entrusted to it by the Central Government from time to time.

1.3 Objectives for Recognition of the Laboratory under the Environment (Protection) Act, 1986 The main objective of recognition of Environmental laboratory under the Environmental Protection Act, 1986, is to develop a laboratory equipped with all the facilities and infrastructure capable to analyze the environmental quality parameters of major concern (prescribed parameters). Since the recognition has been granted in line of the guidelines and the laboratories are notified under the Government of India Gazette following the approved procedure along with periodical surveillance, stands the credibility of these laboratories.

The up-to-date list of Environmental laboratories recognized under The Environment

(Protection) Act, 1986 are posted and updated periodically on the CPCB Website along with its scope, so that the organizations working for various projects in the fields of environment may utilize the services of these recognized laboratories.

1.4 Delegation of powers for Recognition of Environmental Laboratories under the Environment (Protection) Act, 1986 to Central Pollution Control Board (CPCB) by MoEF&CC 2In exercise of the powers conferred under section 23 of the Environment (Protection) Act, 1986 (29 of 1986), the Central Government delegated the powers to CPCB by issuing the Gazette

Notifications with respect to grant of recognition to environmental laboratories as follows: - Gazette Notification No. S.O.145 (E), Dated 21st February, 1991 (Addendum-1), the

powers delegated for recognition of the laboratories are reproduced as below:

1. The Central Government hereby delegates the powers with respect to grant of recognition to laboratories or institutes as environmental laboratories and to appoint or recognize Analysts as Government Analysts, as conferred by clause (b) of sub-section (1) of Section 12 and section 13 respectively of the Environment (Protection) Act, 1986 to the Central Pollution Control Board.

2. Recognition of private laboratories under clause (b) of sub-section (1) of section 12 of the Environment (Protection) Act, 1986 as well as recognition of their Analysts as Government Analysts under section 13 of the Environment (Protection) Act, 1986, will continue to be done by the Central Government.

3. The laboratories recognized under clause (b) of sub-section (1) of section 12 of the Environment (Protection) Act, 1986 shall be specified as Government/ Autonomous/Public Sector Undertaking/Educational Institution/ State or Central Pollution Control Board Laboratories.

Gazette Notification No. S.O. 2340 (E) dated 16th June 2021 (Addendum-2) and amended

Notification S.O. 2409 (E)dated 19th June 2024, The powers delegated for recognition of

the laboratories are as below:

Recognition of environmental laboratories (Govt. & Private) under clause (b) of sub-section

(1) of section 12 of the said act as well as recognition of their Analysts as Government Analysts under section 13 of the Environment (Protection) Act, 1986, shall be done by the Central Pollution Control Board.

1.5 Benefits of Recognition and Accreditation to the Laboratory The Recognition granted to an eligible environmental laboratory signifies that the laboratory has all the major requisites for assessing the quality of environment by analyzing all the concerned parameters under its scope. The recognition also provides many other benefits as

below:  EPA recognition is a mandatory requirement by some of the SPCBs / PCCs for empanelment of laboratories under The Water Act, 1974 and Air Act, 1981.  Recognition/Accreditation is not about who the best is, but identifies the laboratory which has a system of standard procedures with aim to improve the analytical quality and safety.

 The Accreditation/Recognition of labs improves facilitation of accurate analysis, efficiency of treatment and reduction of errors in the laboratory process.  The Recognition and Accreditation increases operational efficiency and productivity.

 Increase of confidence in testing/calibration data and personnel performing work.  Savings in terms of time and money due to reduction or elimination of the need for re- testing of analysts/products.

3 Better control of laboratory operations and feedback to system that the concerned laboratory is technically competent. Thus it boosts reputation of the laboratory among analytical services seekers.  Potential increase in business due to enhanced customer confidence Following paragraphs of this guideline describe the prerequisite, mandatory and other requirements and detailed procedure for seeking recognition under environmental Protection Act, 1986 by any Environmental Laboratory 4CHAPTER 2.0 REQUISITE GUIDELINES FOR APPLICATION FOR RECOGNITIONOF ENVIRONMENTAL LABORATORIES UNDER THE ENVIRONMENT (PROTECTION) ACT, 1986

2.1 Recognition under the Environment (Protection) Act, 1986 The environmental laboratories shall be granted recognition (Fresh/Renewal) under Section 12 and 13 of the Environment (Protection) Act, 1986 for the period of three years with valid accreditation of National Accreditation Board for Testing and Calibration Laboratories Accreditation (ISO/IEC 17025:2017) and certification for Occupational Health and Safety Management System (IS/ISO 45001:2018), under the provisions of the said Act, throughout the recognition period for- a. Fresh or renewal of recognition of laboratory;

b. Changes in the name of Government Analysts; c. Shifting and Name change of laboratory premises.

One month before expiry of validity period, the laboratory has to apply for renewal.

2.2 Who can apply for Recognition - Category of the Laboratory The Environment laboratories of any of the following organizations shall be eligible to apply

for recognition: • Autonomous & Govt. Dept./Institutions including Pollution Control Boards/Committees • Public Sector Undertaking • Educational Institutes (Govt./Govt. Aided/Private) • Private Laboratories • Non Govt. Organization (NGO) • Co-operative Sector Laboratories • Public Private Partnership (PPP) Laboratories • Others

2.3 Application Fees for Recognition The Following fee for processing of applications with respect to grant of recognition to Environmental laboratories under the Environmental (Protection) Act, 1986, has to be paid along with the application by applicant laboratory.

Application Application Fee Fresh Application Rs. 50,000/- + GST as applicable  Applying for the first time (In case application is rejected on account of  Applying after the expiry of not fulfilling mandatory requirement only current recognition 50% of fee will be refunded) 5 Name or Address change of Laboratory Renewal of Recognition/ Changes in the Rs. 25,000/- + GST as applicable name of Govt. Analysts The Laboratories applying for EPA recognition are required to submit fee through payment gateway at the time of filing the application via Web-portal in the designated Account.

2.4 How to Apply for Recognition The environmental Laboratory (Private or Government), seeking recognition (Fresh/Renewal) for the first time, may apply through dedicated Web-Portal specific for Laboratory Recognition under the Environment (Protection) Act, 1986 namely “http://cpcbepalab.in/epalab” available at CPCB Website by filling up all the desired information and submitting the prescribed fee as per the following steps.

Step-1 Registration:

This is the initial step which covers the basic information of laboratory like name, address and contact details of the laboratory. The login ID and the password will be generated in this step by sending SMS or creating one-time password (OTP) for verification at the end of CPCB.

After the login through registered ID, the portal system will take the laboratory to the next

section.

Step-2 Type of recognition:

In this step laboratory has to select the type of recognition for which it is applying for whether new/ renew/ other.

Step-3 Pre-Requisite:

In this step laboratory has to fill/upload all the information as a pre-requisite requirement as mentioned at para 2.9. If the laboratory fails to comply with any of the pre-requisites, the application will not be accepted by the system. This information has to be filled at the time of fresh recognition or first time registration in the portal.

In case laboratory changes its Name, it has to apply afresh as new registration with new name.

Step-4 After completing the first three steps the web-portal will take the laboratory to the new window, where the laboratory has to fill the complete details of laboratory, scope of recognition, quality control activities, video coverage of the lab and photos of laboratory sections with GPS location.

Step-5 The applicant laboratory has to complete the required information at various sections of the application and also upload relevant supporting document as required by the system.

Step-6 6The laboratory has to self-check, whether all the documentation required as in the documentation checklist at para ahead have been uploaded at relevant para of online application. until the entire application is filled up, completed and ready for online submission In case of Renewal, where there is any change in application, laboratory has to follow the steps 4 -

6.

2.5 Duration of Recognition Environment laboratory will be recognized for three years with valid accreditation (ISO/IEC 17025: 2017) and cerification (IS/ISO 45001:2018) during the recognition period.

2.6 Expiry of Recognition The laboratory’s recognition shall not be valid after expiry of recognition period, if application is not submitted for renewal before the expiry of recognition.

2.7 Renewal of Recognition If there is no change with respect to the following, (An undertaking in this regard has to be submitted)  Name and premises of the laboratory  Scope of the recognition  Government Analyst  Instrument/Equipment The laboratory can apply for renewal with the prescribed fee, by submitting the valid renewal certificates of ISO/IEC 17025:2017 (NABL) and IS/ISO 45001:2018, the updated details of CRM, existing manpower and calibration details of instruments/ equipment.

Note: In case there is any change on the above, the laboratory has to submit fresh complete application with prescribed fee.

2.8 Submission of application for Renewal of Recognition Laboratories presently with valid recognition, are advised to apply for renewal of recognition atleast one month before the expiry of their existing recognition with valid ISO/IEC 17025:2017 or IS/ISO 45001:2018 to avoid discontinuation of recognition. The applications submitted after the expiry of recognition shall be treated as a fresh recognition.

2.9 Pre-requisite Requirements for application Laboratories has to fulfil the following pre-requisites applying for the New/ Renewal of Recognition, failing which the laboratory can not move to next step of application:

 Laboratory Legal Entity/ Certificate of incorporation/ Registration/ Authorization  Whether laboratory situated in authorized area  Valid Certificate of Laboratory Accreditation as per ISO/IEC 17025:2017  Valid Certificate of Occupational Health and Safety Management System as per ISO 45001:2018 7 Number of Man-Power: Laboratory has to fulfill minimum requirement of total number of 9 manpower  Area of the Laboratory: The laboratory has to fulfill the criteria of minimum working area requirement of 150 Sq. mt. excluding administration and accounts.

2.10 Complete details of Mandatory Requirements of EPA Recognition

2.10.1 Laboratory Legal Entity/Certificate of Incorporation/ Registration/ Authorization The laboratory (Private/NGOs) under consideration for Recognition under the Environment Protection Act 1986 must be legally identifiable and duly registered with an appropriate statutory body i.e. Local Bodies, State Govt. or Central Govt. For certification of Legal Identity of the laboratory – any one or more of the following documents/Registration Certificate needs to be submitted along with the application for New/Renewal of recognition. The copy of registration certificate must be uploaded while applying through Web-portal. In case laboratory changes its premises or Name, it has to apply afresh with new registration of the new premises/ New Name whatever is the case.

i) Certificate of Registration by Company Registrar and Memorandum of Articles in case the laboratory belongs to a Limited Company ii) Document issued as per Societies Registration Act or Indian Trust Registration Act iii) Partnership Deed and copy of registration under the Limited Liability Partnership Act 2008 iv) In case of Government Body; Gazette or Government Notification or self- Declaration on Letter head by Head of the organization

Note: In case the document(s) of address proof and legal identity is (are) in the local language, then their authenticated English Translation may preferably be provided

2.10.2 Laboratory Site / Location in designated authorized area Laboratory must be situated in designated authorized area as certified by the registering authority. Authenticating the premises of laboratory - any one or more of the following document needs to be submitted i) Certificate from Registrar of Firms or Directorate of Industries or Industries Centre ii) Municipal Corporation / Local Body / MSME/Pollution Control Board or any such Govt. document indicating premises of the applicant laboratory.

2.10.3 Mandatory Accreditation & Certification The laboratory has to submit details of mandatory accreditation as per ISO/IEC 17025:2017 along with its validity as well as for IS/ISO 45001:2018. The copies of these certificates should be uploaded with the application, wherever required. The application received without having mandatory ISO 17025 Accreditation and ISO 45001 Certification will be summarily rejected.

2.10.4 Submission of Detailed Scope of ISO/IEC 17025 Accreditation 8The laboratory has to provide information regarding each parameter whether it is under scope of ISO/IEC 17025:2017 and also a copy of Scope of ISO/IEC 17025:2017 Accreditation depicting various parameters and discipline for which the accreditation has been granted needs to be submitted with the application.

All the critical parameters identified by CPCB for recognition should cover under the scope of ISO/IEC: 17025 Accreditation.

2.10.5 Laboratory Total Work Area The laboratory should have sufficient working area to accommodate all its analytical and supporting activities. There should be total minimum floor space of 150 Sq meter excluding the area allotted to Administration and Accounts as detailed ahead in Table

7.1 Table 2.1 Laboratory Minimum area/Floor space section wise requirement S.No. Laboratory Sections Minimum Space in Sq.mt.

1. Water Analysis Section (Potable water, Ground Water, Natural water (Lakes, Rivers, Wetlands, Estuaries)

2. Waste Water Analysis Section (Sewage, Industrial Effluents, other 60 sq m waste waters)

3. Soil, Sediments, Sludge/Slurry and HW Analysis Section

4. Microbiological & Biological Analysis Section 15 sq. m

5. Ambient Air & Source Emission Monitoring & Analysis Section 25 sq m

6. Instrumentation Section (Common instruments/ equipment and supporting for other analytical sections) 30sq m

7. Instrumentation Section (Sophisticated Instruments )

8. Sampling Equipment, Storage area including compressed gas storage area, Common library, Committee Room & Staff area 20 sq m Total Area 150 sq m Note The dimensions of individual section may vary but Minimum covered area of the Environmental laboratory should not be less than 150 sq. mt.

The applicant environmental Laboratory shall upload its detailed layout plan with dimensions at the time of submitting application for the recognition.

2.10.6 Man-Power-Details The Laboratory must have sufficient skilled manpower according to the Work load of the laboratory. The Manpower employed must be capable of doing specific works allotted to them. The laboratory shall submit the details of all the existing manpower including with the top and senior management as per hierarchy with their Name, Age, Qualifications, Experience details, working at the laboratory since, job responsibilities, emoluments drawn per annum etc. in the format given in Annexure II.

The laboratory should have atleast 09 number with following skilled manpower;

Table 2.2: Indicative list of the Typical Manpower for an Environmental Laboratory 9S. No. Position Qualifications Experience Nature of Work

1. He ad of Ph.D./M.Sc./M.Tech. >10 years Laboratory Management, & Laboratory/Equivalent Supervisory Environmental Supervision & Analysis

2. Ph.D./M.Sc.//B.Tech. >5 years Scientist/Equivalent Junior Scientist M.Sc. (Chemistry/ Instrumental Analysis

3. > 3 years

(Chemical)/Equivalent Environmental Science Junior Scientist M.Sc. (Microbiology/ Microbiological Analysis

4. /E quivalent Environmental >3 years

(Microbiology) Science) Technical Sampling and Analysis M. Sc./B.E./

5. Of ficer/Assistant >3 years B.Tech./Dip. Engg /Equivalent

6. La boratory Analysts M. Sc./B.Sc. .>2 years Laboratory Analysis Laboratory/Field B.Sc. Chemistry, Sampling and Analysis

7. >2 years Assistants /Equivalent Zoology, Botany Laboratory Attendant/ Assistance in Sampling & analysis

8. M ultitasking Staff 12th/Matriculate -

(MTS) /Equivalent Administration / Accounts& Administrative

9. B.Com/BA/BSc >2 years Accounts matters The desired qualification of the personnel involved in the laboratory activities should possesses the below mentioned qualification.

Table 2.3: Recommended qualifications of laboratory and field monitoring personnel:

S. No Activity Minimum Educational Qualification

1. Analysis of Air/ Water Graduates/Post Graduates in Chemistry/Environmental Sciences

2. Analysis of Graduates/Post Graduate in Chemistry/Environmental Soil/hazardous wastes Sciences/ Agricultural Sciences

3. Analysis of Graduates/Post Graduates in Biochemistry/Microbiology/ Microbiological Environmental Sciences/ Agricultural Sciences parameters & Biomonitoring

4. Water & Soil sampling Graduates in Chemistry/Environmental Sciences/Agricultural Sciences/ Graduate/Diploma in Engineering 10+2 with science

5. Air sampling Graduates in Chemistry/Environmental Sciences/ Engineering/ Physical sciences/ Diploma in Engineering

6. Assistance in Laboratory 10+2 with Science/Diploma

7. Field Assistant Diploma in engineering/ITI

2.10.7 Laboratory Organization Structure The applicant laboratory while submitting the application for new/renewal of recognition has to attach the respective Laboratory’s Organization Chart clearly depicting all positions, the hierarchy, the names of present incumbents, analysts and their brief responsibilities with the application.

2.10.8 Work Allotment Details 10The Laboratory has to provide work allotment details of the personnel involved in the various activities of the laboratory in prescribed column of application.

2.10.9 Training Details of Man power The officials involved in various laboratory activities must be well trained and aware with the work assigned to them therefore the laboratory is required to provide the training details of all the laboratory personnel

2.10.10 Code of Ethics & Integrity of Analytical Reports The undertaking for maintaining Code of Ethics & to maintain Integrity of Analytical reports at the laboratory shall be furnished, in the specified format (Annexure I) on the laboratory’s official letterhead duly signed and sealed by the Head of the Organizarion/laboratory, the undertaking may be furnished by the Head of the Laboratory.

2.10.11 GST/PAN Registration The laboratory should have registered under Goods & Services Tax Act and secured GST number, which needs to be mentioned in the application. Similarly, the Income Tax PAN number, also to be indicated in the application.

2.10.12 Geo tagging Details Laboratories are required to provide their location in terms of Latitude and Longitude. For that laboratories are to pin their location in the map provided in the Web-portal to create latitude and longitude for showing exact location of the lab in the map.

2.10.13 Laboratory Photographs Laboratory has to upload the digital photographs of various sections of the laboratory to depict clear picture of the working status of laboratory. All the major analytical and other areas should be covered while taking the photographs. Photographs of following area but not limited to, are to be uploaded on web-portal at the time of submission of application for recognition.

1. Outer View of the laboratory, Building Front Elevation structure

2. Water Analysis Section (Potable water, Ground Water, Natural water (Lakes, Rivers, Wetlands, Estuaries)

3. Waste Water Analysis Section (Sewage, Industrial Effluents, other waste waters)

4. Microbiological & Biological Analysis Section

5. Ambient Air & Source Emission Monitoring & Analysis Section

6. Soil, Sediments, Sludge & Slurry Analysis

7. Wastes (Industrial Solid Waste, Municipal Solid waste) Analysis Section

8. Hazardous Waste Analysis Section

9. Miscellaneous Analysis Section

10. Instrumentation Section

11. Library & Conference/Committee room

12. Laboratory Staff Sitting area

13. Laboratory Waste Collection, Segregation and disposal

14. Gas storage and distribution area

112.10.14 Laboratory Video Clipping The Laboratory has to prepare one short video with geotags covering all its activities relevant to the EPA Recognition (infrastructure, facilities, manpower etc.) and to provide its Google drive link, while submitting the application. Lab video shold not be more than 2 minutes.

2.10.15 Environmental Parameters Analyzed and details of their Analytical Method, Range of analysis, MDL etc.

The laboratory has to provide complete information about various parameters analysed matrix wise, details of their Analytical methods, Range of Analysis, Minimum Detection limit in the relevant column of the application. The complete list of parameters is given at Annexure –IV. The laboratory may apply for all or any of these parameters. However, there are minimum number of analytical parameters are required for Recognition under the Environment (Protection) Act, 1986 under each matrix as per Annexure –III. These parameters have been summarized in the table as given below.

Table: 2.4 ANALYTICAL PARAMETERS FOR RECOGNITION Discipline/ Group: WATER AND WASTEWATER S.No. Category Mandatory parameters W(A) Physical Tests 08 W(B) (i) General& Non-metallic 15 Inorganic (ii) Trace Metals 20 W(C) Organics (General) and Trace Organics 06 (21) W(D) Microbiological Tests 04 W(E) Toxicological Tests 01 Discipline/ Group: SOIL/SEDIMENT:WASTE (Liquid/Slurry/Sludge/Solid/Semi-solid), Plastic waste, Municipal Solid waste S(A) Soil/Sludge/Sediment and Solid Waste 07 S(B) Hazardous Waste Characterization/Analysis 08 Discipline/ Group:AIR A(A) Ambient Air 12 A(B) Stack gases/source emission 09 A(C) Noise level 02 A(D) Meteorological Monitoring 05 Laboratory seeking recognition should compulsorily have comprehensive analytical facilities for all the parameters for which the recognition is applied in a particular group.

Mandatory parameters and applied secondary parameters should be covered under the scope of accreditation as per ISO/IEC 17025: 2017 and recognition shall be granted only for those parameters, which are covered under the scope of accreditation as per ISO/IEC 17025: 2017.

2.10.16 Use of Validated Analytical Methods The analytical testing at the environmental laboratory should be performed by using only validated methods prescribed internationally/nationally are envisaged. While submitting application for Fresh/Renewal of recognition under the Environment (Protection) Act, 1986, the laboratory has to submit details about the methodology used for analysis of respective analytes. The list of parameters with suggestive methods are given at Annexure V.

2.10.17 List of Reference Material/Certified Reference Material 12The laboratory has to fill-up the complete information about availability of various Reference Material (RM) as well as Certified Reference Material (CRM) in the relevant column of the application. The expiry date of each should also be mentioned.

2.10.18 Technical Infrastructure and Equipment The laboratory should have quality infrastructure in conformity with the analytical services requirement expected from a reputed environmental laboratory. The laboratory shall maintain sufficient sampling and sample processing equipment, analytical and support instruments/equipment to conduct required analytical operations. The laboratory shall maintain up-to-date inventory of all instruments/equipment. The laboratories submitting their application of New/Renewal of Recognition under the Environment (Protection) Act 1986 must fill up the information about the availability of various sampling, sample processing and Analytical instruments/equipment (as per Annexure VI&VII) at their laboratory in the prescribed column in the application while submitting the application.

2.10.19 Calibration status of important instruments / equipment with accepted validity While completing information in the application, the laboratory has to provide all the details of instruments and equipment including with range accuracy and calibration details in the relevant column of web-portal with indication of validity of calibration of respective instrument/equipment.

2.10.20 Maintenance of Laboratory Instruments/Equipment The applicant laboratory has to submit details of Instrument/Equipment under warranty, after sales service/ Maintenance contract (AMC, breakdown contract or other type) in the relevant column in the application.

2.10.21 Participation in Inter-Laboratory Analytical Quality Control (IQC) / Proficiency Testing (PT) The laboratories desirous to seek recognition under the Environment (Protection) Act, 1986 shall participate in all inter-laboratory comparison exercise conducted by the Central Pollution Control Board and a proficiency testing programme conducted by the proficiency testing provider certified under ISO/IEC: 17043, covering all the parameters under recognition, within two years from the date of recognition. The Laboratory should achieve Z-Score below 2.0 for all the parameters and the parameters for which the desired score could not be achieved have to be re-participated in the PT through an authorized PT provider and submit the satisfactory report. The performance Report of the past two or three years of PT / ILC Exercise has to be uploaded during filing of the online application for Fresh/Renewal recognition.

2.10.22 Compulsorily Participation in Inter-Laboratory Analytical Quality Control (AQC) conducted by CPCB The environmental laboratories recognized under The Environment (Protection) Act, 1986 have to compulsorily participate in Analytical Quality Control exercises organized by Central Pollution Control Board as per the plan posted on CPCB official website. CPCB may also send environmental samples to the recognized laboratory to keep constant check over the analytical results generated by the recognized laboratory and results will be

provided to the CPCB.The performance of recognized environmental laboratory in 13Analytical Quality Control Exercise should be satisfactory (should not be less than 70%) during the recognition period. In case, the performance of the laboratory has been found below the desired standard, the laboratory has to take immediate necessary action to maintain the analytical quality at the laboratory.

Past three Results of compulsory participation in Analytical Quality Control conducted by CPCB have to be submitted by the laboratory in the application. If the performance of the laboratory is not found satisfactory in any two Analytical Quality Control (AQC) out of the last three AQC in past, in that case, laboratory has to submit Root Cause analysis of parameters (unsatisfactory performance) and also submit satisfactory performance of those parameters by participating in another PT/ILC exercise as per requirement of NABL-163.

2.10.23 CPCB AQC Participation Fees For participation in the CPCB AQC programme, Environmental Laboratories have to submit participation fees in the respective Inter-laboratory AQC Exercise. The fee submission details have to be provided timely to the concerned Laboratory Head at Central Pollution Control Board, Delhi for ensuring the participation in the AQC. (Annexure IX)

2.10.24 Government Analyst A person shall not be qualified for appointment or recognized as a Government analyst unless he or she is possessing following age, qualification and experience.

Age: The Government Analyst under nomination should not have attained the age of the age of 65 yrs or retirement age, whichever is less.

Qualification & Experience: graduation in science from a recognized university with five year experience in environmental investigation, testing and analysis; or Post-graduation in science/ environmental science or a graduate in engineering or a graduate in medicine or equivalent with two years’ experience in laboratory engaged in environmental investigation, testing and analysis.

The applicant laboratory should recommend the names of senior officials involved in analytical operation in the laboratory and fulfilling the criteria for Govt. Analyst in the application proforma in the Web-portal.

The notification of the Government analyst shall deemed be cancelled either he/she leaves the job or the recognition of laboratory gets expired. Laboratory shall immediately inform to CPCB regarding the same and apply for his/ her replacement atleast one month before his/ her leaving the job.

2.10.25 Nomination for proposed Govt. Analysts The laboratory (Pvt. Lab) has to nominate maximum three senior level analysts for consideration as Govt. Analyst at the laboratory as per Section 13 of the Environment

(Protection) Act 1986, in case the laboratory is recognized under the said Act.

2.10.26 Submission of Bio Data by respective nominated Govt. Analyst 14The nominated personnel from the laboratory for consideration of Govt. Analyst have to upload their detailed bio-data/Resume including with training details in the specified format at Annexure XII for consideration of Recognition under The Environment

(protection) Act 1986. The documentary evidences of qualification, experience, training obtained in the field of sampling and analysis of environmeal samples has to be submitted along with the biodata.

2.10.27 Terms & Conditions for Recognition of Government Analyst The analysts recommended by the laboratory in the application will be considered and selected by CPCB as Government Analyst. These selected analysts will have to submit Acceptance of Terms &Conditions by Govt. Analyst unconditionally, as specified in the application as per Annexure XI.

2.10.28 Substitution of Govt. Analyst during the period of recognition The Laboratory should appoint their analyst atleast for the period of validity of Recognition. Even though, if any of the recognized Govt. analysts by CPCB, vacates his position due to superannuation / death / resignation from the services of the concerned laboratory, the recognized laboratory may nominate substitute and if found qualified as per laid guidelines will be accepted and notified as Govt. Analyst.

2.10.29 Authorized Signatory of Analysis Reports issued by recognized lab The analysis report depicting analytical results of environmental samples will be issued by the recognized environmental laboratory under signature of concerned analyst, supervisor analyst and have to be countersigned by any one of government analysts designated by the Central Govt or authorized signatory of NABL. The Government analyst recognized with the environmental laboratory will be the authorized signatory of analysis reports generated by the concerned laboratory.

2.10.30 Acceptance of Terms & Conditions for Recognition of Laboratory under the Environment (Protection) Act 1986 by Head of Laboratory While submitting application for Fresh/Renewal of recognition under the Environment

(Protection) Act, 1986, the laboratory has to upload acceptance of Terms & Conditions for Recognition of Laboratory under the Environment (Protection) Act 1986 in the specified format as given at Annexure X.

2.10.31 Bio-Metric Attendance System Laboratories are required to install Bio-Metric Attendance system at the entrance of Laboratory to ensure the daily attendance of all the employees as well as the Govt. Analysts to provide the Biometric attendance information of all the laboratory officials for past two months.

2.10.32 Laboratory Waste generation (Liquid waste, Solid waste, Spent Solvents, Bio- hazardous waste) collection and disposal practices The laboratory has to submit the write up about approximate quantity of various types of Laboratory waste generated, their segregation, adequate collection and disposal practices.

2.10.33 Other Requirements for Best Laboratory Practices 15Laboratories have to furnish details of following requirements, whenever asked or at the time of Surveillance visit/ audit or surprise inspection.

 Electricity Supply Load and Power Back up (DG set & UPS system) Information of Electric supply load for the laboratory operation, Power back up arrangement such as Diesel Generator set with its capacity and details of UPS system their capacities and nos.

 Laboratory Environmental Condition, Air Handling Unit & HVAC system Details of AHU & HVAC system their capacity/tonnage  Water Supply & Distilled / Deionized Water source The laboratory should have provision for continuous raw water supply either from a direct supply source or through storage tanks. Details of water supply whether bore well water, piped water or water supplied through tankers. Similarly, the details of Distilled/deionized water availability through in house water purification system or glass distillation system or water softening system or quantity of deionized water purchased from the supplier to be mentioned.

 Quality of Glassware/Labwares and Chemicals used Information about the makes/quality of Glassware/Labwares and Chemicals.  Record of Evidence for Intra Laboratory Analytical Quality Control The applicant Laboratory will have to adopt all the steps and procedures of Intra laboratory Analytical Quality Control required for checking the precision and accuracy of analytical results within laboratory but not limited to as per the clause

7.7.1 of the ISO 17025 and systematically maintain its record.  Use of Laboratory Signage & Symbols The laboratory should adopt and provide the globally harmonized Laboratory signage and symbols, wherever these are required, within the laboratory operation area.

 Laboratory Safety/Protection Equipment The laboratory should provide information in the relevant para in the application about the list of available numbers of various Laboratory safety and protection equipment.

List of few such equipment is given at Annexure-VIII  Maintenance of Customer Service Feedback, Complaints and their Resolution The laboratory should have adequate mechanism for Customer Service feedback, to register their appreciation as well as complaints and procedure for the resolution of complaints and should be shown to the inspection/ surveillance team at the time of visit.

2.11 Documentation Checklist for fulfilment of requirement of Recognition-Documents Checklist for completeness of Application (New/Renewal) for Recognition of the Laboratory under The Environment (Protection) Act, 1986 16S. Requirements as per the Guidelines for Recognition of Laboratory under the E (P) No. Act 1986

1. Legal identity of Laboratory Certificate of Incorporation / Registration / Authorization

2. GST/PAN Registrations

3. Location details of the laboratory in Authorized Area (indicate in Google Map)

4. Video clip of laboratory (link) to be provided by the laboratory

5. Laboratory Covered Area, dimensions of various sections Laboratory Layout Map showing different sections

6. Laboratory Photograph including with External Photograph with front elevation of building

7. Undertaking by Laboratory Head to follow Code of Ethics in the specified format

8. Laboratory Organization Structure/Chart

9. Details of Senior Management of Laboratory List of total Manpower with Qualifications, Designation, Experience, Work responsibilities and Present Emoluments

10. Biometric Attendance of all laboratory officials (past three months)

11. Work allotment Details of Laboratory Personnel

12. Training Details/Record of Laboratory Personnel

13. Write up/SOP of Laboratory Waste Management Practices

14. List of Sampling Instruments/Equipment

15. List of sample processing Instruments / Equipment

16. List of Analytical Instruments, details of their date of purchase, Authorization for operation

17. Calibration status of important instruments / equipment with accepted validity.

18. Details of Maintenance of Instrument/Equipment List of Instrument/Equipment Calibration, and Maintenance Service Contracts

19. Water Supply & Distilled/deionized water

20. Quality of Glassware/Labware and Chemicals used including with their brands and grading

21. Environmental Parameters Analyzed and details of their Analytical Method, Range of analysis, MDL etc

22. List of Reference Material/Certified Reference Material

23. Record of Evidence for Intra Laboratory Analytical Quality Control

24. Record of Evidence for External AQC/PT participation during preceding three years

25. Past three Results of compulsory participation in Inter-laboratory Analytical Quality Control conducted by CPCB

26. Copy of Certificate for NABL Accreditation (17025:2017) along with detailed Scope with accepted validity at least 1½ years prior from the expiry date

27. Copy of ISO 45001 Certification Certificate with validity & Expiry

28. Copy of Scope of ISO 17025 Accreditation highlighting the mandatory parameters required for The E(P)Act 1986 recognition included in the scope

29. Acceptance of Terms & Conditions for Recognition of Laboratory under the Environment

(Protection) Act 1986

30. Nomination of Proposed Govt Analysts (Maximum three)

31. Bio data of proposed Government Analysts in prescribed proforma with photograph, records of qualification, Training, Experience, Salary, etc.

32. Acceptance of Terms & Conditions by the nominated personnel for consideration of Govt.

Analysts

33. Latest Schedule of Analytical charges, levied by the laboratory for various analysis, which should not exceed as notified by CPCB

34. Any other relevant documents in support of application

2.12 Submission of Duly Completed Application 17As per the clause 2.4, how to apply for recognition after the acceptance of pre-requisite from the system the Application format for Recognition has to be completed online. After filling up all the required information online as per requirement of EPA Guidelines, preparing all the enclosures as per the information sought beforehand the duly completed application have to be uploaded through the Web portal. The successful payment of Application Fees as applicable has also to be submitted online,

2.13 Amendments/changes with application proforma and evaluation criteria If felt necessary CPCB may amend / change application proforma and criteria.

2.14 Scrutiny /processing of Application The applications received from applicant laboratories for consideration of recognition through Web-portal will be scrutinized at first instance by concerned section of CPCB as per the criteria laid down in the guidelines. If the application is found with major shortcoming related with infrastructural requirement or not fulfilling the application will liable to be rejected and laboratory has to apply afresh after fulfilling the desired requirement.

2.15 Communication of shortcomings in the infrastructure facilities of the laboratory If minor deficiencies have been found in the proposal with respect to laid down criteria for recognition, the same will be communicated to concerned laboratory by CPCB with detailed statement of deficiencies for clarification/rectification through web-portal within the defined time limit, failing which the laboratory has to apply for Fresh recognition.

2.16 Rejection of Application (if found incomplete or non - fulfilment of pre-requisite criteria).

If the laboratory fails to fulfill the pre-requisite or mandatory requirements as per this requirement the application will be rejected and only 50 percent of application fee will only be refunded. The laboratory has options to apply fresh, once it fulfills all the requirements

2.17 Meeting all the requirements as per the Checklist on Web-portal When the Laboratory finds complying with all the requirement of this document before or after taking all the action taken within the stipulated time limit against the shortcomings as communicated to the laboratory the compliance status of the application is forwarded to Competant Authority for approval .

2.18 Grant of Recognition and Gazette Notification The Environmental Laboratory and Govt. Analyst found qualified, recommended by the Expert Committee, approved by the competent authority, and finally on unconditional acceptance of Terms & condition by the laboratory may be Gazette notified in Govt. of India Gazette

2.19 Expert Committee 18Expert Committee has been constituted at CPCB to review the procedure of recognirion of laboratory as and when required. The Expert Committee will comprise of members from (i) MoEF&CC (ii)State Pollution Control Board (iii) NABL (iv) NEERI (v) CPCB Expert, (vi) CPCB Member Convener

2.20 Procedure for Processing and Evaluation of Environmental Laboratory for Consideration of Recognition under the Environment (Protection) Act 1986 As per the revised Guidelines to grant recognition to Private laboratories as well as Government laboratories under the Environment (Protection) Act 1986, the procedural steps

for evaluation of the application are summarized below:

Procedural Steps followed for Processing of Application Step-I Scrutiny / Processing of the application based on the criteria for Evaluation & Assessment of Application submitted for recognition by CPCB Team, Step-II Verification of the information, documents and all the requirement as submitted in the application form by CPCB Team as detailed in various paras at Chapter 2.0.

Step-III Status of the application (Accepted / Rejected) with the observations/comments of CPCB Team will be reflected on web-portal.

Step-IV In case of the incomplete information in the application, the application will be send back to the laboratory for completing the information.

Step-VI Verification of the information/corrective actions resubmitted by the applicant and complete application with comments shall be forwarded to for final recommendation.

Step-V Based on the recommendation of CPCB Team, approval from the Competent Authority of CPCB for consideration of Environmental Laboratory for Recognition under the Environment (Protection) Act 1986.

Step-VI Generate and issue the Recognition Certificate after approval.

Step-VII Gazette Notification of approved environmental laboratory and Govt.

Analysts.

Step VIII The list of approved recognized Environmental laboratories under the Environment (Protection) Act 1986 will be posted on CPCB Website (www.cpcb.nic.in).

2.21 Time limit for Disposal of application After receiving of complete application with all the desired documents /information on the web-portal, maximum one month shall be required to get it approved by the Competent Authority.

2.22 Use of Phrase “Recognized under the Environment (Protection) Act, 1986” The recognized environmental laboratory under The Environment (Protection) Act, 1986 may use the phrase “Recognized under The Environment (Protection) Act, 1986” on the analysis report, official stationary, signboards etc. However, the validity period of recognition has also to be mentioned alongwith the phrase.

2.23 Obligation of the Laboratory during the period of Recognition 19The laboratories recognized under The Environment (Protection) Act, 1986 will have jurisdiction to function as Environmental laboratory in any state of the country. However, sample collection, preservation and transport protocol will have to be adopted and maintained by the laboratory as per standard references.

2.24 Desktop Audit, Surveillance, and Surprise Inspection of recognized laboratories Laboratory should willingly ready and affirm any surprise surveillance/inspection either physically or virtually, by the joint inspection team of MoEF&CC, CPCB and SPCB, any time during the period of recognition. In case, joint inspection team find/observe any major shortcomings, hiding of facts by laboratory, inadequate supporting documents for respective analytical work undertaken, or laboratory reportedly indulged in gross manipulation of analytical data may lead to revoking/withdrawal of recognition granted.

2.25 Inspection Team The joint inspection team of MoEF&CC, CPCB and SPCB will comprise of atleast two/three officers having experience in the field.

2.26 Actions to be taken against non-compliance Following actions may be taken against the non-compliance/ Shortcomings found by the inspection team during the visit.

Reversal of action after taking corrective action S. No. Shortcomings Action to be taken by the laboratory

01. Pre-requisite Revoking of recognition To apply a fresh after requirements fulfilling the requirement Mandatory Requirements

02. Use of standard/ Recognition for concerned To submit the documentary Validated Analytical parameter(s) shall be evidence after the standard/

Methods: cancelled validated method adopted.

03. Availability of Until the valid CRM is To submit certificate of Reference purchased the analysis of valid CRM.

Material/Certified concerned parameters shall be Reference Material with stopped accepted validity

04. Technical Infrastructure Revoking of recognition if To apply a fresh after

and Equipment: any Instrument / equipment fulfilling the requirement not available for mandatory parameter(s) Recognition for concerned To submit supporting parameter(s) shall be document after fulfilling cancelled if shortcoming is the requirement for secondary parameter

05. Calibration status of Until the valid Calibration To submit valid calibration important instruments / certificate is submitted, certificate. equipment with accepted analysis of concerned

validity: parameters shall be stopped

2006. Participation in Inter- Suspension of Recognition if To participate in the PT/ Laboratory Analytical not participated in the PT AQC for the said parameter Quality Control (AQC) / exercises for mandatory and submit satisfactory Proficiency Testing parameters report

(PT): Recognition for concerned parameter(s) shall be cancelled if shortcoming is for secondary parameter

07. Compulsorily Suspension of Recognition if To participate in the same Participation in Inter- not participated in at least 2 parameter/s from any Laboratory AQC out of last 3 AQCs conducted authorized PT Provider conducted by CPCB by CPCB with satisfactory Recognition shall be performance and send the withdrawn for those report unsatisfactory parameters in the last 3 AQC for which corrective action was not taken

08. Qualification and The laboratory shall be To submit record of newly competency of derecognized till the appoint Govt. Analyst and Government Analyst laboratory appoints another the revival of recognition competent Govt. Analyst with will be based on accepted qualification verification of records

09. Training Details/Record The analyst may be stopped To submit training record of Analysts for for analysis till he/she gets the for the concerned analyst performing proper training

Environmental Analysis:

10. Photographs provided The recognition shall be To apply afresh application with the application not suspended at least for 3 after 3 months matching with the months on false reporting original location

11. Complaints On verification of the After taking corrective complaint, if it is found to be action the laboratory will correct and/or any violation intimate to CPCB and or non-compliance apply a fresh after committed by the laboratory, verification from CPCB.

the recognition shall be cancelled for 3 months

2.27 Penalty for contravention of the provisions of the Act and rules, orders and directions Any Such activity which fails to comply with the or contravenes any of the provisions of the Act or Rules made or orders or directions issued there under penalties may be imposed on the laboratories as per the Environment (Protection) Act, 1986;

2.28 Show Cause Notice & Revoking of Recognition The CPCB reserves its right to de-recognize/revoke its recognition or issue show cause notice at any time in public interest without assigning any reason, if it is deemed necessary by the Central Govt./CPCB. The recognition will also be revoked during following events:

 In case, the laboratory indulges in malpractices and issuing fraudulent reports.

21 There are complaints against the laboratory regarding analytical malpractices.  The laboratory violates the accepted terms & conditions for recognition of environmental laboratory under the Act.  The laboratory not complying the rules and regulations notified under The Act.

22Annexure I UNDERTAKING TO FOLLOW CODE OF ETHICS I……………………………………………………………………………the Laboratory Head / authorized signatory of Environmental Laboratory of M/s____________________________________________________________________ Address_________________________________________________________________ _______________________________________________________________________ Submitting application for recognition under The Environment (Protection) Act, 1986 as we understood to have fulfilled the Laboratory Recognition mandatory and optional Criteria as stipulated by Central Pollution Control Board / Ministry of Environment, Forest & Climate Change do hereby undertake that:

1. Integrity: We shall have a professional behaviour, be straightforward and honest in all professional and business practices and maintain absolute integrity of analytical data/information.

2. Objectivity: We shall not allow bias, conflict of interest or undue influence of others including clients in our professional testing and reporting activity.

3. Professional competence and due care: It is our continuing duty to maintain professional knowledge, skill and testing facilities at the level required to ensure that the customer receives competent professional services based on current technological advancements, revision/amendment in the analytical procedures, standards etc.

4. Our laboratory will not indulge in deliberate falsification of analytical results, where failed method requirements are made to appear acceptable during reporting. An intentional gross deviation from specified analytical method and practices, combined with the intent to conceal the true analytical value

5. The following practices shall be strictly discouraged:  Sharing of test results with the customer/client before completion of testing as well as before final issue of report.  Reporting the test results without proper/actual testing on the environmental sample  The intentional recording or reporting of incorrect analytical information  Giving any undue favor to the customer/client in terms of testing and reporting of results

6. Confidentiality: We shall maintain adequate confidentiality with respect to the sample under test and the test results.

7. Professional behaviour: We shall comply with relevant laws and regulations and should avoid any action that discredits the profession :

Date : Laboratory Head / Authorized Signatory Place Name & Designations

Seal of Laboratory: _________________________________________________________________

Note: The undertaking shall be furnished, signed and sealed by the Proprietor or Director or Partner of the applicant laboratory on laboratory’s official stationery (letter-head). For Government sector labs, the undertaking may be furnished by the senior most head of the Lab.

23Annexure II Details of Laboratory Personnel at the Laboratory as per Organization chart Total Working at Emoluments experience the drawn per S. Name Designation Qualifications (years & laboratory annum No months) Since Top Management Officials Laboratory Personnel Training attended in the field of environmental sampling and analysis and quality control systems may be included 24Annexure III LIST OF MANDATORY ANALYTICAL PARAMETERS FOR RECOGNITION Discipline/ Group:

WATER AND WASTEWATER

(A) Physical Tests:

S. No. Parameter

1. Temperature

2. Colour

3. pH

4. Turbidity

5. Conductivity

6. Total solids

7. Total dissolved solids (TDS)

8. Total suspended solids (TSS)

(B) Inorganic (i) General& Non-metallic S. No. Parameters

1. Alkalinity

2. Chloride

3. Cyanide

4. Dissolved oxygen

5. Nitrite nitrogen

6. Nitrate nitrogen

7. Ammonical nitrogen

8. Fluoride

9. Hardness (Total)

10. Calcium

11. Magnesium

12. Phosphate

13. Sulphate

14. Sulphide

15. Total Residual chlorine (TRC)

(ii) Trace Metals S. No. Parameter

1. Aluminium (Al)

2. Arsenic (As) Total

3. Barium

4. Boron

5. Chromium (Cr) Hexavalent

6. Chromium (Cr) Total

7. Cadmium (Cd)

8. Cobalt (Co)

9. Copper (Cu)

10. Iron (Fe)

2511. Lead (Pb)

12. Manganese (Mn)

13. Mercury (Hg)

14. Nickel (Ni)

15. Potassium (K)

16. Sodium (Na)

17. Vanadium (V)

18. Zinc (Zn)

19. Selenium (Se)

20. Sodium Adsoption Ratio (SAR)

(C) Organics (General) and Trace Organics S. No. Parameters

1. Biological Oxygen Demand (BOD)

2. Chemical oxygen demand (COD)

3. Oil & Grease

4. Phenolic Compounds as C H OH 6 5

5. Benzopyrene

6. Pesticides

6.a. Organochlorine Pesticides (OCPs) i. Aldrin ii. Alpha Endosulphan iii. p,p’-DDT iv. Alpha-HCH v. Beta HCH vi. Beta Endosulphan vii. Gama-HCH viii. o,p’-DDT ix. p,p’-DDE

6.b. Organophosphorus Pesticides (OPPs) i. Malathion ii. Methyl parathion iii. Chloropyriphos iv. Dimethoate v. Dieldrin vi. Ethion

(D) Microbiological Tests S. No. Parameters

1. Total Coliform

2. Faecal Coliform

3. E. coli

4. Faecal Streptococci

(E) Toxicological Tests S. No. Parameter

1. Bioassay method for evaluation of toxicity using fish (90% survival of fish after 96 hrs in 100% effluent) Discipline/ Group: SOLID/SOLIDWASTE 26(A) Soil/ Sediment /compost S. No. Parameter

1. Cation Exchange Capacity (CEC)

2. Electrical Conductivity (EC)

3. Organic carbon (chemical method)

4. pH

5. Soil moisture

6. Total nitrogen

7. Metals by digestion (As, Cd, Cr, Pb, Ni etc.)

(B) Hazardous Waste (Liquid/Slurry/Sludge/Solid//Semi-Solid) S. No. Parameter

1. Corrosivity

2. Ignitability (Flash point)

3. Loss on Drying at 1050C (% Moisture content)

4. Loss on Drying at 5500C (% Organic content)

5. pH

6. Organic carbon/matter (chemical method)

7. Calorific Value

8. Toxicity Characteritics leaching procedure (TCLP) Laechate and Metals in Laechate ((As, Cd, Cr, Pb, Ni) Discipline/ Group:AIR

(A) Ambient Air S. No. Parameters

1. Nitrogen dioxide as NO 2

2. Sulphur dioxide (SO 2)

3. Particulate matter (PM 10)

4. Particulate matter (PM 2.5)

5. Carbon Monoxide

6. Ozone

7. Benzene

8. Ammonia

9. Metals in Particulate Matter, Pb

10. Metals in Particulate Matter, As

11. Metals in Particulate Matter, Ni

12. Particulate Benzo-a-Pyrene (BaP)

(B) Stack Gases/Source Emission S. No. Parameter

(i) Mandatory Parameters

1. Particulate Matter

2. Sulphur Dioxide

3. Carbon Dioxide

4. Carbon Monoxide (NDIR based Method)

5. Temperature

6. Moisture

7. Oxygen

8. Oxides of Nitrogen

9. Halides (HCL/HF)

(C) Noise Level 27S. No. Parameters

1. Ambient Noise level measurement (20 to 140 dB(A)

2. Source Noise Level Measurement ( Industrial Process, DG Set etc ) (20 to 140 dB(A)

(D) Meteorological Monitoring S. No. Parameters

1. Ambient Temperature

2. Wind direction

3. Wind speed

4. Relative Humidity

5. Mixing Height 28Annexure IV List of all the other parameters may be applied for Recognition S. No. Physical Tests

01. Salinity

02. Odour

03. Flocculation test (Jar test)

04. Fixed Volatile solids

05. Settleable solids

06. Sludge volume index (SVI)

07. Velocity & discharge Measurement of industrial effluent stream Inorganic (i) General& Non-metallic

01. Acidity

02. Bromide

03. Chlorine demand

04. Free chlorine

05. Dissolved Carbon dioxide

06. Iodide

07. Silica

08. Sulphite

09. Total Kjeldhal Nitrogen (TKN)

10. Total Phosphorus Trace Metals

01. Beryllium (Be)

02. Antimony (Sb)

03. Lithium (Li)

04. Selenium (Se)

05. Silver (Ag)

06. Strontium (Sr)

07. Tin (Sn)

08. Thalium (Tl) Organics (General) and Trace Organics

01. Total Organic Carbon (TOC)

02. Adsorbable Organic Halide (AOX)

03. Surfactants

04. Tannin & lignin

05. Polynuclear aromatic hydrocarbon (PAH) a. Naphthalene b. Acenaphthalene c. Acenaphthylene d. Phenanthrene e. Anthracene f. Fluoranthene g. Pyrene/ Benzo(a) pyrene

06. Carbon/Nitrogen ratio

07. Poly-chlorinated biphenyl (PCB’s) as enviro indicator a. 2,4,4’-trichlorobiphenyl (PCB-28) b. 2,2’,5,5’-tetrachlorobipheny (PCB-52) c. 2,2’,4,5,5’-pentachlorobiphenyl(PCB-101) d. 2,3’,4,4’,5-pentachlorobiphenyl(PCB-118) e. 2,2’,3,4,4’,5’-hexachlorobiphenyl(PCB-138) f. 2,2’,4,4’,5,5’-hexachlorobiphenyl(PCB-153) g. 2,2’,3,4,4’,5,5’-heptachlorobipheny (PCB-180)

8. Phenolic Compounds 29a. Phenol b. 4-nitrophenol c. 2,4-dinitrophenol d. 2-nitrophenol d. 2-chlorophenol e. 2,4-dimethylphenol f. 2-methyl,4,6-dinitrophenol g. 4-chloro,3-methylphenol h. 2,4-dichlorophenol i. 2,4,6-trichlorophenol j. Pentachlorophenol Microbiological Tests

01. Total plate count

02. Coliphage Toxicological Tests

01. Measurement of toxicity factor using zebra fish (dimensionless toxicity test

02. Bio-accumulation, bio magnification and bio-transformation studies

03. Estimation of the effect at tissue level

04. Measurement of toxicity using Daphnia or other organism Biological Tests

01. Diversity Score (Benthic macro-invertebrates)

02. Benthic organism identification and count

03. Macrophytic identification

04. Planktonic identification count

05. Measurement of various diversity index

06. Saprobity Index

07. Chlorophyll

08. Primary productivity

09. P/R Ratio Soil/Sludge/Sediment and Solid Waste

01. Available N, P, K

02. Micronutrients-Available Fe, Cu, Mn, Zn Mo By DTPA

03. Available Boron

04. Available Sulphur

05. Exchangeable Ca, Mg, Na

06. Sodium Absorption Ration (SAR)

07. Exchangeable sodium percentage (ESP)

08. Gypsum requirement

09. Lime Requirement

10. Metals in Soil by digestion

11. Persistent Organic pollutants, Pesticides and Insecticides

12. Soil Texture

13. Soil Structure

14. Total Soluble Sulphates as SO 4

15. Total Soluble Solid

16. Water holding capacity (WHC) Hazardous Waste Characterization/Analysis

01. Cyanide, total and amenable to chlorination

02. Nitrate

03. Reactivity

04. Metals in waste (Sb, Be, Se, Te, Th, Co Cu, Mo, Sn, W, V, Ag, Ba, Zn, Hg, Hexavalent chromium)

05. Persistent Organic pollutants, Pesticides and Insecticides

06. Phenols

3007. Sulphide

08. Toxicity

09. TOC

10. Toxicity characteristic leaching procedure (TCLP) and Metals in Laechate

11. Toxicity characteristic leaching procedure (TCLP) and Persistant Organic Pollutant, Pesticides and Insecticides in Laechates

12. Hydrogen cyanide released from wastes

13. Hydrogen sulphide released from wastes

14. Paint filter Liquid Test

15. Liquid Release Test Municipal Solid Waste/Plastic Waste

01. Composition and characterization (% by weight)

02. Density

03. Moisture

04. N-P-K (Total Nitrogen) (Phosphorus as P O ) (Potassium as K O) 2 5 2

05. C/N ratio

06. Biochemical Characteristics: carbohydrates, proteins, natural fiber and biodegradation factor

07. Degradation of Plastics

08. Toxicity profile of MEW included heavy metals, oersistant organic pollutants, presticides, and insecticides

09. Thichness of Plastic Ambient Air

01. Hydro sulphide

02. Anions in Particulate Matter, Bromide

03. Anions in Particulate matter, Chloride

04. Anions in Particulate matter, Fluoride

05. Anions in Particulate matter, Nitrate

06. Anions in Particulate matter, Nitrite

07. Anions in Particulate matter, Phosphate

08. Anions in Particulate matter, Sulphate

09. Cations in Particulate matter, Ammonium

10. Cations in Particulate matter, Barium

11. Cations in Particulate matter, Calcium

12. Cations in Particulate matter, Lithium

13. Cations in Particulate matter, Magnesium

14. Cations in Particulate matter, Potassium

15. Cations in Particulate matter, Sodium

16. Cations in Particulate matter, Strontium

17. Metals in Particulate Matter, Cd

18. Metals in Particulate Matter, Cr

19. Metals in Particulate Matter, Cu

20. Methane

21. Non Methane Hydrocarbon

22. Polycyclic Aromatic Hydrocarbon (PAH)

23. Volatile Organic Carbon

24. Carbonyls (Formaldehyde & Acetone)

25. Polychlorinated Dibenzo-Dioxins (PCDDs)

26. Polychlorinated Dibenzofurans (PCDFs) Stack Gases/Source Emission

01. Sulphuric Acid mist (As SO ) 3

02. Chlorine

03. Total Organic Carbon

04. Hydrogen Sulphide

05. Carbon Disulphide

06. Mercaptan

3107. Polychlorinated Dibenzo-Dioxins (PCDDs)

08. Polychlorinated Dibenzofurans (PCDFs) Fugitive Emissions

01. SPM

02. BTEX and other VOCs

03. Methane

04. H S 2

05. Metals (Pb, Ni, As, Cu, Cd, Cr, Sb)

06. Hydrocarbons

07. Total Organic Compounds Noise level

01. DG Set Noise Leq Meteorological Monitoring

01. Solar radiation

02. Rain fall

03. Atmospheric Pressure

Note:Any other sample , not listed in the above list may be considered under the Secondary parameters.

32ANNEXURE V Analytical Methods As stipulated in ISO/IEC 17025, clause 7.2 the analytical testing at the environmental laboratory should be performed by using only validated methods prescribed internationally/nationally are envisaged. Following are the prescribed methods to be adopted by the laboratories for designated parameters;

A. Methods for Analysis of Water, Wastewater and Groundwater For waters, wastewaters and ground waters, methods selected from the standard references listed below should be used.

International:

1. American Public Health Association (APHA), Standard Methods for the Examination of Water and Wastewater. Latest Edition.

2. US Environmental Protection Agency SW846 Online, Methods for Chemical Analysis of Water and Wastes, www.epa.gov/epawaste/hazard/testmethods/sw846/online/index.htm#table

3. American Society for Testing and Materials (ASTM), Water and Environmental Technology.

4. US Environment Protection Agency 1978, Microbiological Methods for Monitoring the Environment, Water and Wastes.

National

1. Bureau of Indian Standard (BIS), Drinking Water Standard IS 10500:2018

2. IS 3025: Part 1: 2021. Methods of sampling and test (Physical And Chemical) for water and

wastewater: Part 1 sampling (First Revision) ·

3. IS: 3025. (Various Parts i.e. from Part 2 to Part 80) Method of Sampling and Test (Physical and Chemical) for Water and Wastewater B. Trace Metals Analysis in Water, Waste water, Soil, Sediments, Solid and Hazardous waste.

The summary of various instrumental techniques used for trace metals analysis in environmental matrices are presented in Table 5.2 :

Table 5.1 Methods (International) for Trace Metal Analysis in Environmental matrices S. Element Environmental Range of Methods Instrument No. matrices analysis 1 Calcium In all matrix Parts per Million APHA 3500-Ca B

(ppm) IS 3025 2 Magnesium In all matrix Parts per Million APHA- 3500-Mg B --

(ppm) IS 3025 3 Sodium Water and Waste Ratio USEPA-SW 846 Absorption water 1S11624 -- Ratio (SAR) Soil/ Sediment USEPA-6020A, 7000B 33S. Element Environmental Range of Methods Instrument No. matrices analysis In all Matrix Parts per Million APHA 4500 B, 4500 B-C 4 Boron (ppm) IS 3025 Spectrophotometer Drinking water, Parts per Million APHA 3500-Cr B, 5 Chromium Surface Water and (ppm) 3500- Cr C Spectrophotometer/ Hexavalent Ground Water 1S 3025 Ion Chromatograph Wastewater and Parts per Million Industrial discharge (ppm) etc.

6 Sodium, In all Matrix Parts per Million APHA 3500 NaB, Potassium (ppm) 3500- K B Flame Photometer IS 3025 7 Cadmium, Drinking water, Parts per Billion APHA, 3111, 3113,3120 Selection of Chromium (T), Surface Water and (ppb) to Parts per and 3125. instruments (AAS, Copper Ground Water Million (ppm). ICP-OES and ICP- Iron, Lead, Wastewater and Parts per Billion APHA, 3111, 3120 and MS etc.) as per Nickel, Zinc Industrial discharge (ppb) to Parts per 3125. parameter-wise etc. Million (ppm). applicable methods.

Soil, Sediment, Solid Parts per Billion USEPA 3050-B, 6010, waste and Hazardous (ppb), Parts per 6020 and APHA, 3125.

Waste etc. Million (ppm) and % level.

8. Arsenic Water and wastewater Parts per Billion APHA, 3113, 3114,3120

(ppb) to Parts per and 3125.

Million (ppm).

Soil, Sediment, Solid Parts per Billion USEPA 3050-B, 6010, waste and Hazardous (ppb) to Parts per 6020 and APHA, 3125.

Waste etc. Million (ppm).

9 Mercury Water, Wastewater Parts per Billion APHA, 3112 and 3125. AAS and ICP-MS or and Industrial (ppb) to Parts per equivalent dedicated discharge etc. Million (ppm). instruments.

Soil, Sediment, Solid Parts per Billion EPA 7471, 7473 and waste and Hazardous (ppb) to Parts per 6020.

Waste etc. Million (ppm).

10 Aluminium, Drinking water, Parts per Billion APHA, 3111, 3113,3120 Beryllium, Surface Water and (ppb) to Parts per and 3125. AAS, ICP-OES and Barium, Ground Water Million (ppm). ICP-MS.

Lithium, Wastewater and Manganese, Industrial discharge Selenium, etc.

Strontium, Soil, Sediment, Solid Parts per Billion USEPA 3050-B, 6010, Tin, Antimony, waste and Hazardous (ppb), Parts per 6020 and APHA, 3125.

Cobalt, Waste etc. Million (ppm) and Vanadium % level.

Indian Standard Methods

1. Bureau of Indian Standards, IS 10500 (2018): Drinking Water with latest amendments.

2. IS 3025 (Part 2):2004 Method of Sampling and Test (Physical and Chemical) for Water and Wastewater, Determination of 33 Elements by Inductively Coupled Plasma Atomic Emission Spectroscopy

Remarks: The BIS methods documented for Spectrophotometric analysis of heavy metals/trace metals are mostly not suitable for environmental matrices analysis as they are only for analysis in PPM and percentage range, while in environment the presence of trace metals are expected in PPB and Sub PPB levels, 34C. Trace Organics in Water, Waste Water, Soil, Sediments, Solid waste and Hazardous waste The suggestive analytical methods to be followed for analysis of these compound groups in environmental matrices are presented in the Table 5.2 below:

Table 5.2: Trace Organics Analytical Methods in various Environmental Matrices (Water, Waste water, Soil and Sediments).

S. Trace Organic Instrument Analytical Method No. Compounds Mandatory Trace Organics Analysis Organochlorine pesticides Gas Chromatograph with capillary USEPA Methods – 508 & 8081 1 (HCH, DDT, Aldrin, column and Electron Capture using GC-ECD Endosulphan) Detector (ECD) USEPA Method 608 Organophosphorus/Organon Gas Chromatograph with capillary USEPA Methods – 507 & itrogen pesticides column and Nitrogen Phosphorus 8141B using GC-NPD / GC- 2 (Malathion, methyl Detector (NPD) / Flame photometric FPD parathion, chlorpyriphos) Detector (FPD) Gas Chromatograph with capillary USEPA Methods – 3540, column and Electron Capture 3610, 3630, 3660, 8082 & 3 Polychlorinated Biphenyls Detector (ECD) / Mass Spectrometer 8280 using GC-ECD / GC-

(PCBs) Detector (MSD) LRMSUSEPA Method 608 High Pressure Liquid Chromatograph

(HPLC) with UV-VIS / UV-DAD Detector or Gas Chromatograph with USEPA Method 610 4 Polyaromatic hydrocarbons capillary column and Flame

(PAHs) Ionisation Detector (FID) / Mass Spectrometer Detector (MSD) Purge & Trap System (Aqueous Samples) or Head Space System USEPA Method – 524.2 Volatile Organic (Solid Samples), using Purge & Trap with 5 Compounds (VOCs) Gas Chromatograph with capillary GC-LRMS column and Mass Spectrometer USEPA Method 1624 Detector (MSD) Gas Chromatograph with capillary USEPA Method –501.1 Trihalomethanes (THMs) column and Electron Capture (Archive method) 6 Detector (ECD) / Mass Spectrometer US EPA Method – 551.1 Detector (MSD) High Pressure Liquid Chromatograph USEPA Method – 531.1 7 Carbamates (HPLC) with UV-VIS / UV-DAD USEPA Method – 632 Detector USEPA Method – 632.1 Gas Chromatograph with capillary column and Electron Capture USEPA Method – 515.1 8 Herbicides Detector (ECD) or High Pressure USEPA Method – 8151A Liquid Chromatograph (HPLC) with USEPA Method – 8321B UV-VIS / UV-DAD Detector D. Microbiological Analysis Microbiological analysis in environmental samples is performed through a range of test methods designed for that purpose.The suggestive analytical methods to be followed for analysis of microbiological parameters in environmental matrices are presented in the Table 5.3 below:

Table 5.3: Microbiological Analysis in environmental samples of Drinking water, Natural waters and waste waters S. Parameter Method Method Reference 35No.

1. Total coliform and a. Multiple tube technique b. Membrane filter technique American Public Health

2. Faecal Coliform a. Multiple tube technique Association (APHA), b. Membrane filter technique Standard Methods for the

3. Faecal streptococci a. Multiple tube technique Examination of Water and Wastewater. Latest Edition. b. Membrane filter technique

4. Enterococcus a. Multiple tube technique Bureau of Indian Standards; b. Membrane filter technique (BIS) Methods for Microbiological examination a. Pore plate method of water

5. Total plate count b. Spread plate method c. Membrane filter method 6 E. Coli a. Multiple tube technique b. Membrane filter technique Indian Reference Methods (BIS):

1. IS 1622: 1981 Methods of sampling and Microbiological Examination of water

2. IS 15188:2012 Water quality — General guide to the Enumeration of micro-organisms by culture

3. IS 5401 (Part 1): 2012 Microbiology – General guidance for enumeration of coliforms; Part 1 Colony count technique (first revision)

4. IS 15185: 2016 Water Quality — Detection and Enumeration of Escherichia coli and Coliform bacteria — Membrane FiltrationMethod

5. IS 15188:2012 Water quality — Horizontal method for the detection of Enumeration of coliforms; Part 1 Colony count technique (first revision) E. Toxicological and Biological Analyses Prescribed methods for toxicological analysis in environmental samples are mentioned in table

5.4 below:

Table 5.4 Methods for Toxicological Analysis in Environment S.

Toxicological Test Reference method No Bioassay method for evaluation of American Public Health Association (APHA),

1. toxicity using fish (90% survival of Standard Methods for the Examination of Water and fish after 96 hrs in 100% effluent) Wastewater. Latest Edition.

IS 6582 (Part 1) 1971 Bio-Assay Methods for Evaluating Acute Toxicity of Industrial Effluents and Waste Waters 2 Measurement of toxicity factor IS 6582 : (Part 2) : 2001 (Reaffirmed Year : using zebra fish (dimensionless 2019)Bio-assay methods for evaluating acute toxicity toxicity) test of industrial effluents and wastewaters : Part 2 Using Toxicity Factor to Zebra Fish 3 Measurement of toxicity using Daphnia or other organism 364 Bio-accumulation, bio-magnification American Public Health Association (APHA), and bio-transformation studies Standard Methods for the Examination of Water and Wastewater. Latest Edition.

Bio-monitoring of biological parameters has significant advantages over traditional analysis of abiotic matrices (water, sediments). Besides providing information on the bio availability of contaminantsFor Biological Monitoring or Bio-monitoring following are the important studies undertaken apart from toxicological studies.

Table 5.5 Methods for Bio-Monitoring/ Biological Analysis parameters in the Environment S.No Biological Monitoring Reference Method

1. Benthic macro- American Public Health Association (APHA), 2017 Standard invertebrates sample Methods for the Examination of Water and Wastewater. 23rd collection Edition; Benthic macro-invertebrates part 10500; PP 10-67 to 10-81

2. Benthic macro- invertebrates identification D. de Zwart and R.C. Trivedi, 1995Manual on Integrated Water and count Quality Evaluation, CPCB Report 3 Measurement of Diversityindex 4 Measurement of Saprobity Index 5 Macrophytic identification American Public Health Association (APHA), 2017 Standard 6 Planktonic identification Methods for the Examination of Water and Wastewater. 23rd count Edition;

7 Chlorophyll A) Aggregate Organic Constituents, Part 5000; P5-1;

8 Primary Productivity Inorganic non-metallic constituents Part 4500, P4-114 9 P/R Ratio B) Archieve.epa.gov/wattarchieve/web/html/ch03main.h tml Other References

1. USEPA, 2002Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms Fifth Edition.

2. USEPA, 2002 Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms Fourth Edition.

3. USEPA, 2021 Elements of Bio Monitoring/Bio Assessment, Chapter 3.

F. Hazardous Waste Analysis Analytical methods are required to be standardized for characterization of wastes specialized training for the concerned personnel is also required.

Table 5.6 Hazardous waste Characterization Analysis at Hazardous Waste at Environmental laboratories S. Hazardous waste Characteristics Reference method No. Test Ignitable wastes can create fires under certain conditions, are spontaneously combustible, or have USEPA 1010-1986

1. Ignitability USEPA 1020-1986 37a flash point less than 60 °C (140 °F). Examples include waste oils and used solvents Corrosive wastes are acids or bases (pH less than or equal to 2, or greater than or equal to 12.5) that are USEPA SW-846 Test capable of corroding metal containers, such as Method 1110A:

2 Corrosivity storage tanks, drums, and barrels. Battery acid is an Corrosivity toward Steel example Reactive wastes are unstable under "normal" USEPA40 CFR Section conditions. They can cause explosions, toxic fumes, 261.23 includes 8 different 3 Reactivity gases, or vapors when heated, compressed, or mixed properties of a waste with water. Examples include lithium-sulphur identifying characteristics batteries and explosives of reactivity.

Toxic wastes are harmful or fatal when ingested or absorbed When toxic wastes are disposed, the toxic USEPA 1311-1992 & constituents may leach from the waste and pollute APHA 2017 -3120B 4 Toxicity ground water. Example include wastes containing mercury, lead, DDT, PCBs, etc.

Toxicity Characteristics Leaching Procedure (TCLP) 1-USEPA SW-846 Method 1311 Toxicity Characteristic Leaching Procedure 2-Testing Procedure for Analysis of Hazardous Waste Samples, SCHEDULE II [See rule 3 (1) (17)

(ii)] of HOWM Rules, 2016.

Table 5.7 Hazardous Waste Constituents, Concentration limits and Analytical Methods

Class A: Based on leachable concentration limits [Toxicity Characteristic Leaching Procedure (TCLP) or Soluble Threshold Limit Concentration (STLC)] Class Constituents Concentrati Analytical Method on (mg/L) A1 Arsenic 5.0 ICP Method (APHA 3120 B) AAS Method ( APHA 3114B) A2 Barium 100.0 AAS Method APHA 3111B ) AAS Method APHA 3120 B) A3 Cadmium 1.0 ICP Method (APHA 3120 B) AAS Method (APHA 3111B) A4 Chromium and/ or 5.0 ICP Method (APHA 3120 B) Chromium (III) AAS Method (APHA 3111B) A5 Lead 5.0 AAS Method (APHA 3111B) ICP Method (APHA 3120B ) A6 Manganese 10.0 ICP Method (APHA 3120 B) AAS Method (APHA 3111B) A7 Mercury 0.2 ICP Method (APHA 3120 B) AAS Method (APHA 3112B) A8 Selenium 1.0 ICP Method (APHA 3120 B) AAS Method (APHA 3114B) A9 Silver 5.0 ICP Method (APHA 3120B) A10 Ammonia 50* Distillation followed by Titrimetric Method (APHA 4500- NH B/C) 3 A11 Cyanide 20* Distillation followed by Titrimetric Method (APHA 4500-CN- C/D:) A12 Nitrate (as nitrate- 1000.0 UV-Vis Screening Method (APHA 4500-NO3-B nitrogen) A13 Sulphide (as H2S) 5.0 Iodometric Method (APHA 4500-S2- F ) A14 1,1-Dichloroethylene 0.7 38A15 1,2-Dichloroethane 0.5 USEPA Method 8260 B Volatile Organic A16 1,4-Dichlorobenzene 7.5 Compounds byGas Chromatography Mass A17 2,4,5-Trichlorophenol 400.0 Spectrometry (GC-MS) A18 2,4,6-Trichlorophenol 2.0 A19 2,4-Dinitrotoluene 0.13 A20 Benzene 0.5 A21 Benzo (a) Pyrene 0.001 USEPA Method 8310: Poly nuclear Aromatic Hydrocarbons A22 Bromodicholromethane 6.0 USEPA Method 524.2 Measurement of A23 Bromoform 10.0 Purgeable organic compounds in water by A24 Carbon tetrachloride 0.5 Capillary Column Gas chromatography/Mass Spectrometry (GC-MS) A25 Chlorobenzene 100.0 USEPA Method 8260 B Volatile Organic Compounds byGas Chromatography Mass Spectrometry (GC-MS) A26 Chloroform 6.0 USEPA Method 524.2 Measurement of Purgeable organic compounds in water by Capillary Column Gas chromatography/Mass Spectrometry (GC-MS) A27 Cresol (ortho+ meta+para) 200.0 USEPA Method 8260 B Volatile Organic Compounds byGas Chromatography Mass Spectrometry (GC-MS) A28 Dibromochloromethane 10.0 USEPA Method 524.2 Measurement of Purgeable organic compounds in water by Capillary Column Gas chromatography/Mass Spectrometry (GC-MS) A29 Hexachlorobenzene 0.13 USEPA Method 8081B Organochlorine A30 Hexachlorobutadiene 0.5 Pesticides by GasChromatography A31 Hexachloroethane 3.0 A32 Methyl ethyl ketone 200.0 NIOSH Method 2500 by Gas Chromatography A33 Naphthalene 5.0 USEPA Method 8310: Polynuclear Aromatic Hydrocarbons A34 Nitrobenzene 2.0 Gas Chromatography Mass Spectrometry Method (APHA6410B : 2012) A35 Pentachlorophenol 100.0 USEPA Method 8260 B Volatile Organic A36 Pyridine 5.0 Compounds byGas Chromatography Mass A37 Tetrachloroethylene 0.7 Spectrometry (GC-MS) A38 Trichloroethylene 0.5 A39 Vinyl chloride 0.2 A40 2,4,5-TP (Silvex) 1.0 USEPA Method 8321B Herbicides by High A41 2,4- 10.0 Pressure LiquidChromatography (HPLC) Dichlorophenoxyacetic acid A42 Alachlor 2.0 USEPA Method 8081B Organochlorine A43 Alpha HCH 0.001 Pesticides by GasChromatography A44 Atrazine 0.2 Method 8141B: Organophosphorus Compounds by GasChromatography A45 Beta HCH 0.004 USEPA Method 8081B Organochlorine A46 Butachlor 12.5 Pesticides by GasChromatography A47 Chlordane 0.03 A48 Chlorpyriphos 9.0 USEPA Method 8141B: Organophosphorus Compounds byGas Chromatography A49 Delta HCH 0.004 USEPA Method 8081B Organochlorine A50 Endosulfan (alpha+ 0.04 Pesticides by GasChromatography beta+sulphate) A51 Endrin 0.02 USEPA Method 8141B: Organophosphorus A52 Ethion 0.3 Compounds byGas Chromatography A53 Heptachlor (& its 0.008 USEPA Method 8081B Organochlorine Epoxide) Pesticides by GasChromatography 39A54 Isoproturon 0.9 USEPA Method 532 by Gas Chromatography A55 Lindane 0.4 USEPA Method 8081B Organochlorine Pesticides by Gas Chromatography A56 Malathion 19 USEPA Method 8141B: Organophosphorus Compounds byGas Chromatography A57 Methoxychlor 10 USEPA Method 8081B Organochlorine Pesticides by Gas Chromatography A58 Methyl parathion 0.7 USEPA Method 8141B: Organophosphorus A59 Monocrotophos 0.1 Compounds byGas Chromatography A60 Phorate 0.2 A61 Toxaphene 0.5 USEPA Method 8081B Organochlorine Pesticides by Gas Chromatography A62 Antimony 15 ICP Method (APHA 3120 B) AAS Method (APHA 3112B) A63 Beryllium 0.75 ICP Method (APHA 3120 B) AAS Method (APHA 3114B) A64 Chromium (VI) 5 . 0 Colorimetric Method (APHA 3500-Cr- B) IC method (APHA 3500-Cr- C) A65 Cobalt 80.0 AAS Method (APHA 3111B) ICP Method (APHA 3120B) A66 Copper 25.0 ICP Method (APHA 3120 B) AAS Method (APHA 3111B) A67 Molybdenum 350 AAS Method (APHA 3111D) A68 Nickel 20.0 AAS Method (APHA 3111B) ICP Method (APHA 3120B) A69 Thallium 7.0 ICP Method (APHA 3120 B) AAS Method (APHA 3111B) A70 Vanadium 24.0 AAS Method (APHA 3111B) ICP Method (APHA 3120B) A71 Zinc 250 ICP Method (APHA 3120 B ) AAS Method (APHA 3111B) A72 Fluoride 180.0 SPANDS method (APHA 4500-F- D) A73 Aldrin 0.14 USEPA Method 8081B Organochlorine A74 Dichlorodiphenyltrichloro 0.1 Pesticides by Gas Chromatography ethane (DDT), Dichlorodiphenyldichloro ethylene (DDE), Dichlorodiphenyldichloro ethane (DDD) A75 Dieldrin 0.8 A76 Kepone 2.1 USEPA Method 8260 B Volatile Organic Compounds by Gas Chromatography Mass Spectrometry (GC-MS) A77 Mirex 2.1 USEPA Method 8081B Organochlorine Pesticides by Gas Chromatography A78 Polychlorinated biphenyls 5.0 USEPA Method 8082A Polychlorinated Biphenyls by Gas Chromatography A79 Dioxin (2,3,7,8-TCDD) 0.001 USEPA Method 1613B Tetra- through Octa- Chlorinated Dioxins and Furans by Isotope Dilution by HRGC-HRMS USEPA Method 8081B Organochlorine Pesticides byGas Chromatography

Class B: Based on Total Threshold Limit Concentration (TTLC) Class Constituent Concentration Test method (mg/Kg) 40B1 Asbestos 10,000 USEPA method 100.1 Analytical Method for Determinationof AsbestosFibers inwater B2 Total 5,000 USEPA Method 8015CNon- PetroleumHydrocarbons halogenated Organics by

(TPH)(C5 - C36) GasChromatography (C6 – C28)

Note:

The testing method for list of constituents at A1 to A61 in Class-A, shall be based on Toxicity Characteristic Leaching Procedure (TCLP) and for extraction of leachable constituents, USEPA Test Method 1311 shall be used.

The testing method for list of constituents at A62 to A79 in Class- A, shall be based on Soluble Threshold Limit Concentration (STLC) and Waste Extraction Test (WET) Procedure given in Appendix II of section 66261 of Title 22 of California Code regulation (CCR) shall be used.

In case of ammonia (A10), cyanide (A11) and chromium VI (A64), extractions shall be conducted using distilled water in place of the leaching media specified in the TCLP/STLC procedures*.

A summary of above specified leaching/extraction procedures is included in manual for characterization and analysis of hazardous waste published by Central Pollution Control Board and in case the method is not covered in the said manual, suitable reference method may be adopted for the measurement.

In case of asbestos, the specified concentration limits apply only if the substances are in a friable, powdered or finely divided state.

The hazardous constituents to be analysed in the waste shall be relevant to the nature of the industry and the materials used in the process.

Wastes which contain any of the constituents listed below shall be considered as hazardous, provided they exhibit the characteristics listed in Class-C of this Schedule:

Class-C S. No. Constituent Test Methods

1. Acid Amides USEPA METHOD 8316 Acrylamide, Acrylonitrile and Acrolein by High Performance Liquid Chromatography (HPLC)

2. Acid anhydrides USEPA METHOD 8270D

3. Amines Semi-volatile Organic Compounds by Gas

4. Anthracene Chromatography/Mass Spectrometry (GC/MS)

5. Aromatic compounds other than those listed in Class-A

6. Bromates, (hypo-bromites) USEPA METHOD 300

7. Chlorates (hypo-chlorites) Determination of Inorganic anions in Drinking Water by Ion Chromatography

8. Carbonyls USEPA METHOD 8315A Determination of Carbonyl Compounds by HPLC

9. Ferro-silicate and alloys

10. Halogen- containing USEPA METHOD 9023 compounds which produce Extractable Organic Halides (EOX) in Solids acidicvapours on contact with humid air or water e.g. silicontetrachloride, aluminum chloride, titanium tetrachloride

11. Halogen- silanes

12. Halogenated Aliphatic USEPA METHOD 8121 Compounds Chlorinated Hydrocarbons by

Gas Chromatography: Capillary Column Technique

13. Hydrazine (s)

14. Hydrides

15. Inorganic Acids USEPA METHOD 9056A Determination of Inorganic Anions by Ion 41S. No. Constituent Test Methods Chromatography

16. Inorganic Peroxides

17. Inorganic Tin Compounds

18. Iodates

19. (Iso- and thio-) Cyanates

20. Manganese-silicate

21. Mercaptans

22. Metal Carbonyls USEPA METHOD 207-2

Analysis for Isocyanates By HPLC:

23. Metal hydrogen sulphates

24. Nitrides

25. Nitriles USEPA METHOD 8316 Acrylamide, Acrylonitrile and Acrolein by HPLC

26. Organic azo and azooxy USEPA METHOD 8321B Compounds Solvent-Extractable Non-volatile Compounds by High-Performance Liquid Chromatography/ Thermospray/Mass Spectrometry (HPLC/TS/MS) Or Ultraviolet (UV) Detection

27. Organic Peroxides ASTM E298 Standard Method for Organic Peroxides

28. Organic Oxygen Compounds

29. Organic Sulphur Compounds

30. Organo- Tin Compounds USEPA METHOD 8323 Determination of Organotins by Micro-Liquid Chromatography- Electrospray Ion Trap Mass Spectrometry

31. Organo nitro- and nitroso USEPA METHOD 8270D compounds Semi-volatile Organic Compounds by Gas Chromatography/Mass Spectrometry (GC/MS)

32. Oxides and hydroxides except those of hydrogen carbon, silicon, iron,aluminum, titanium,manganese,magnesium, calcium

33. Phenanthrene USEPA METHOD 8270D Semi-volatile Organic Compounds by GC/MS

34. Phenolic Compounds USEPA METHOD 8041A Phenols by Gas Chromatography

35. Phosphate compoundsexcept USEPA METHOD 365.3 phosphates ofaluminum, calcium Phosphorous, all forms andiron

36. Salts of pre-acids

37. Total Sulphur

39. Tellurium and tellurium compounds

40. White and Red Phosphorus USEPA Method 7580

41. 2-Acetylaminofluorene White Phosphorus (P4) by Solvent Extraction and USEPA METHOD 8270D

42. 4-Aminodiphenyl Semi-volatile Organic Compounds by GC/MS

43. Benzidine and its salts

44. Bis (Chloromethyl) ether 42S. No. Constituent Test Methods

45. Methyl chloromethyl ether

46. 1,2-Dibromo-3-chloropropane

47. 3,3'-Dichlorobenzidine andits salts

48. 4- Dimethylaminoazobenzene

49. 4-Nitrobiphenyl

50. Beta-Propiolactone USEPA METHOD 8260C Volatile organic compounds by GC/MS G. Soil, Sludge, Sediment and Solid Waste Analysis Soil analysis is a set of various chemical processes that determine the chemical, physical, and biological soil properties important for plant nutrition, or “Soil health” Soil contamination, soil pollution, or land pollution as a part of land degradation is caused by the presence of xenobiotic chemicals or other alteration in the natural soil environment. It is typically caused by industrial activity, agricultural chemicals or improper disposal of waste.

There are several standards methods issued by various organizations world over, which may be standardized and adopted at the environmental laboratory for analysis of soil, sediments and solid waste. Following are some of standard references for analytical methods for analysis of Soil, Sludge, Sediment and Solid Waste.

International:

1. American Society for Testing and Materials (1992): Standard test method for determination of the composition of unprocessed municipal solid waste. ASTM Method D 5231 – 92

(September)

2. ASTM D8064 Standard Test Method for Soil and Solid Waste

3. USEPA 1999 Characterization of Municipal Solid Waste in the United States Internet: www.epa.gov

4. Environmental Protection Agency EPA Ireland (1996): Municipal Waste Characterization, ISBN 1 899965 32 7, Ireland

5. EPA Method 3050B: Acid Digestion of Sediments, Sludges, and Soils

Indian:

1. Bureau of Indian Standard (BIS), IS: 2720 (Part 1) - 1983. Indian Standard. Methods Of Test For Soils. Part 1 Preparation. Of Dry Soil. Samples for various Tests. (Second Revision)

2. Bureau of Indian Standard (BIS), IS: 2720 (various parts such as Part 2 to 9) - 1983. Indian Standard Methods of Test for Soils. (2ndRevision) Table 5.8 Ambient Air (Manual & Continuous Analysers), Indoor Air & Fugitive Emission Analysis S.No. Methods of Analysis Analytes Chemical Method Continuous Analyzers

1. Sulphur Dioxide (SO2),  Improved West and Gaeke  Ultraviolet fluorescence μg/m3

2. Nitrogen Dioxide (NO2),  Modified Jacob  Chemiluminescence μg/m3 &Hochheiser (Sodium- Arsenite) 3 Particulate Matter (size less than 10 μm) or PM10  Gravimetric  TOEM μg/m3  Beta attenuation 434 Particulate Matter (size  TOEM less than 2.5  Gravimetric  Beta attenuation microns) or PM

2.5 μg/m3 5 Ozone (O3) μg/m3  Chemical method  UV photometric  Chemiluminescence 6 Lead (Pb) μg/m3  AAS / ICP method after sampling on EPM 2000 or equivalent filter paper _  ED – XRF using Teflon filter

7. Carbon Monoxide (CO)  Non Dispersive Infra Red mg/m3 (NDIR) Spectroscopy

8. Ammonia (NH 3) μg/m3  Indophenol blue method  Chemiluminescence

9. Benzene (C6H6) μg/m3  Adsorption and desorption  Gas followed by GC analysis chromatographybased Continuous analyser

10. Benzo (a) Pyrene (BaP) –  Solvent extraction followed particulate phase only by HPLC / GC analysis ng/m3 _

11.  AAS / ICP method after sampling on EPM 2000 or Arsenic (As) equivalent filter paper AAS / _ ng/m3 ICP method after sampling on EPM 2000 or equivalent filter paper

12.  AAS / ICP method after sampling on EPM 2000 or Nickel (Ni) ng/m3 equivalent filter paper AAS / _ ICP method after sampling on EPM 2000 or equivalent filter paper International Methods

1. Methods of Air Sampling and Analysis, 2020 , By James P. Lodge Jr, 3rd Edition June 30, 2020 by CRC Press, 784 Pages

2. USEPA Procedures for Collecting Ambient Air Samples, Ambient Air Sampling (3/28/2016, SESDPROC-303-R5)

3. Handbook of Air Toxics - Sampling, Analysis, and Properties, Lawrence H. Keith, Mary Walker, CRC Press, 640 Pages Indian Methods

1. CPCB Guidelines “Methods for Measurement of Air Pollution- Guidelines for Manual Sampling and Analysis, National Ambient Air Quality Series: NAAQMS/36/2012-13

2. CPCB Methods and Standard Operating Procedures of Emission Testing in Hazardous Waste Incinerator, LATS series

3. IS 5182IS 5182: Part 1: 2006Methods for measurement of air pollution may 2006: Part 1 dust fall (First Revision)

4. IS 5182: Part 2: 2001 (1 Revision) Methods for measurement of air pollution: Part 2 sulphur dioxide (First Revision)

5. IS 5182: Part 2: Sec 2: 2018Methods for measurement of air pollution: Part 2 sulphur dioxide:

Sec 2 ultraviolet fluorescence method

446. IS 5182: Part 4: 1999 (1 Revision) Methods for measurement of air pollution: Part 4 suspended - Particulate matter (First Revision)

7. IS 5182: Part 5: 2020 (1 Revision) Methods for Measurement of Air Pollution Part 5 Sampling of Gaseous Pollutants (First Revision)

8. IS 5182: Part 6: 2006 (1 Revision) Method for measurement of air pollution: Part 6 oxides of nitrogen (First Revision)

9. IS 5182: Part 6: Sec 2: 2018Methods for measurement of air pollution: Part 6 oxides of

nitrogen: Sec 2 chemiluminescence method

10. IS 5182: Part 7: 2021 (1 Revision) Methods For Measurement of Air Pollution Part 7 Hydrogen Sulphide FIRST REVISION

11. IS 5182: Part 10: 1999 (1 Revision) Methods for measurement of air pollution: Part 10 carbon monoxide (First Revision)

12. IS 5182: Part 11: 2006 (2 Revision) Methods for measurement of air pollution: Part 11 benzene, toluene and xylene (BTX) (Second Revision)

13. IS 5182: Part 12: 2004 (1 Revision) Method for measurement of air pollution: Part 12 Polynuclear Aromatic Hydrocarbons (PAHs) in air particulate matter (First Revision)

14. IS 5182: Part 13: 1991Methods of measurement of air pollution: Part 13 total fluorides in ambient air

15. IS 5182: Part 15: 1974Methods for measurement of air pollution: Part 15 mass concentration of particulate matter in the atmosphere

16. IS 5182: Part 15: Sec 2: 2018/ISO 10473: 2000Methods for Measurement of Air Pollution Part 15 Mass Concentration of Particulate Matter Section 2 Beta-ray absorption method

17. IS 5182: Part 19: 2022(1 Revision) Methods For Measurement Of Air Pollution Part 19 Chlorine First Revision

18. IS 5182: Part 20: 1982Methods for measurement of air pollution: Part carbon disulphide

19. IS 5182: Part 21: 2001Methods for measurement of air pollution: Part 21 non methane hydrocarbons in air by gas chromatography

20. IS 5182: Part 22: 2004Methods for measurement of air pollution: Part 22 lead

21. IS 5182: Part 23: 2006Methods for measurement of air pollution: Part 23 respirable suspended particulate maiter (PM 10), cyclonic flow technique

22. IS 5182: Part 24: 2019Methods for Measurement of Air Pollution Part 24 Fine Particulate Matter (PM )

2.5

23. IS 5182: Part 25: 2018Methods for measurement of air pollution: Part 25 ammonia

24. IS 5182: Part 26: 2020 Method For Measurement of Air Pollution Part 26 Nickel H. Source Emissions Sampling & Measurement The source emission monitoring requirements can necessitate periodic manual monitoring/measurements or through Continuous Emission Monitoring Systems (CEMS) related to consent terms or conditions (e.g., emission limits, work practice requirements, equipment design and operating requirements) that result from regulations. For Manual Source Emission Monitoring following international or Indian Emission measurement methods have to be adopted for systematic monitoring.

Table 5.9 Source Emission Sampling & Measurement S.No. Source Emission Test Method Sampling & measurement

1. Composition of Flue Gas & Molecular Weight Determination

452. Stack Gas Static pressure determination 3 Stack gas velocity& temperature determination USEPA Test 4 Stack gas volumetric fl ow/Discharge Methods 5 Flue Gas Moisture determination 6 Stack Sampling Port and Traverse point CPCB Emission 7 Sulfur Dioxide in Emissions Regulations 8 Sulphuric Acid Mist 9 Nitrogen Oxides in Emissions Bureau of Indian 10 Particulate Matter (PM) emissions Standard (BIS) 11 Hydrogen Halides and Halogens Methods of 12 Metals and Non Metals in Emissions Measurement of Stationary Sources International Methods

1. USEPATest Method 1— Sample and Velocity Traverses for Stationary Sources(40 CFR Part 60, Appendix A) 4-28-2020

2. USEPA Test Method 2 –Determination of Stack Gas Velocity and Volumetric Flow Rate- S-type Pitot, 8-2-2017

3. USEPA Test Method 3 – Gas Analysis for Determination of Dry Molecular weight

4. USEPA Test Method 4—Determination of Moisture Content in Stack Gases 10-7-2020.

5. USEPA Test Method 5 Determination of particulate matter emissions from stationary sources 12-7-2020

6. USEPA Test Method 6 Determination of Sulfur dioxide emissions from stationary sources 8-3-2017

7. USEPA Test Method 7 Determination of Nitrogen oxide emissions from stationary sources 1-14-2019

8. USEPA Test Method 8 Determination of SO2 and sulphuric acid mist

9. USEPA Test Method 10 Determination of Carbon Monoxide- NDIR

10. USEPA Test Method 29 Determination of metals emissions from stationary sources 8-2- 2017

11. USEPA Test Method 201A Determination of PM10 and PM2.5 Emissions From Stationary Sources (Constant Sampling Rate Procedure) 10-7-2020 Indian Methods

1. Guidelines on Methodologies for Source Emission Monitoring” Central Pollution Control Board, LATS / 80 / 2013-14

2. CPCB Emission Regulation (Part III) Comprehensive Industry Document Series COINDS/20/1984-85

3. CPCBGuidelines for Continuous EmissionMonitoring Systems (CEMS), July 2017

4. IS 11255 : Part 1 : 1985Methods for measurement of emissions from stationary sources: Part 1 particulate matter

5. IS 11255 : Part 2 : 1985Methods for measurement of emissions from stationary sources: Part 2 sulphur dioxide

6. IS 11255 : Part 3 : 2008(1 Revision)Methods for measurement of emissions from stationary

sources: Part 3 flow rate (First Revision)

7. IS 11255 : Part 4 : 2006(1 Revision)Method for measurement of emission from stationary

sources: Part 4 hydrogen sulphide and carbon disulphide (First Revision)

8. IS 11255 : Part 5 : 1990Methods of measurement of emissions from stationary sources: Part 5 total fluoride

469. IS 11255 : Part 6 : 1999Methods of measurement of emissions from stationary sources: Part 6 ammonia

10. IS 11255 : Part 7 : 2005Methods for measurement of emission from stationary sources: Part 7 oxides of nitrogen

11. IS 17133 : 2019/ISO 10396 : 2007Sampling from Stationary Sources for Automated Determination of Gas Emission Concentration Using Permanently Installed Monitoring Systems I. Noise/Sound level Monitoring Noise pollution is unwanted and unpleasant sound which can deteriorate human health and other living organisms present in the Environment. Noise or sound level monitoring or measurement is a process to measure the magnitude of Noise in residential, commercial and industrial areas.

Data collected from Noise level helps us to understand trends and action required to be taken to reduce noise pollution.

Ambient Noise Level Ambient Noise measurement includes Residential area noise measurements, Noise measurement in vicinity of Road traffic, Industrial Noise or Noise generated within Industry.

Source Generated Noise level Source generated Noise is the loud sound generate continuously from a defined source, running machinery noise, hammering, drilling, heavy duty motors, lathe machine work, grinding, fabrication, forging, compressing, breaking, pumping, packing, transporting etc. The intensity of noise generated is quite high and may cause serious and large scale Noise related problems for workers and those people who are in vicinity Table 5.10 Noise Measurement S.No. Noise measurement parameters Method

1. Noise Level, dBA Measurement by Sound Level Meter

2. Equivalent Continuous Sound Pressure Level (L ) Department of Environment and EQ Resource Management (2000) 3 Sound Exposure Level (LAE or SEL) “Noise Measurement Manual”, 3rd 4 Percentile sound pressure level exceeded for Edition,Queensland, Australia.

90% of the measurement period, (L90) 5 Percentile sound pressure level exceeded for IS-9989:1981·Assessment of Noise 50% of the measurement period, (L50) with respect to Community Response J. Plastic waste Management Rules – Plastic carry Bag Thickness measurement The Union Ministry of Environment, Forest & Climate Change (MoEFCC) has notified the Plastic Waste Management Amendment Rules, 2021, ondated 12th August 2021 that makes it mandatory for the thickness of plastic carry bags to be increased to 120 microns by December, 2022, and prohibit the manufacture, import, stocking, distribution, sale and use of several products with low utility but high littering potential.The permitted thickness of the plastic bags, 50 microns, has been increased to 75 microns from September 30, 2021, and will be increased to 120 microns from the December 31, 2022, according to the gazette notification. The basic behind the polybags measurements is that the larger the micron/gauge/millimeter of polybag, the thickness of the polybag increases.

47Table 5.11 Plastic carry Bag Thickness measurement S.No.

Method Plastic Poly bags

1. Plastic Poly Bag Thickness Digital Micro Meter Method Non-Contact Capacitance Thickness Gauge IS:2508-1984 Specifications for Low Density Polythene Films ASTM D6988 – Guide for Determination of Thickness of Plastic Film Test Specimens ASTM D8136 – Standard Test Method for Determining Plastic Film Thickness and Thickness Variability Using a Non-Contact Capacitance Thickness Gauge

2. Bio-degradability ISO-14855-2, 2018-07; Determination of the ultimate aerobic biodegradability of plastic materials under controlled composting condition – Method by analysis of evolved carbon di-oxide 48ANNEXURE VI List of Sampling Instruments/Equipment at Environmental Laboratory S. No. Name of Instruments/ Nos. S. No. Name of Instruments/ Nos.

Equipment Availa Equipment Availa ble ble Sampling Equipment/items- Water (Ground water, River water & Lake water);

Waste water (sewage, industrial effluents)

1. Antiseptic Hand wash / soap 23 Ice packs

2. BOD/DO Bottles 24 Measuring Tape 25 mtrs

3. Borosilicate Glass Sample bottles 25 Nylon Rope 20 mtrs (Transparent & Amber coloured

4. Bottom Sampler 26 Oil & Grease sampler

5. Buckets (Stainless steel) 10 litres 27 Packing tape separate for water and waste water sampling

6. Chloroscope for residual chlorine 28 Permanent Marker Pen Black/Blue

7. Depth sampler 29 pH strips/pH meter pen type

8. Disposable Gloves & Rubber 30 Plastic Beaker/plastic Mug / gloves Volumetric Cylinder

9. Dissolved Oxygen meter hand 31 Plastic bucket held type

10. Dissolved Oxygen Sampler 32 Plastic Storage bags

11. Distilled Water Bottle 33 Polypropylene Bottles

12. Drinking water storage insulated 34 Polypropylene/Polyethylene Jug Carboys (various capacities)

13. Electrical Conductivity meter pen 35 Sampling Rod / Bamboo stick type maximum 2.5 mtrs length

14. Field Aprons 36 Self-stick Cotton Tape for Labelling of carboys/bottles

15. Field Multiparameter Water 37 Stainless steel Mug Testing Kit

16. Field Notebook 38 Stainless steel scale

17. First Aid Kit 39 Stop Watch

18. Float Balls (For Flow 40 Thermometer measurement

19. Flow meter 41 Turbidity Meter pen type

20. GPS 42 Wash Bottles

21. Gum Boots & Waders 43 Water sampler, Vandorn or equivalent (Automatic sampler when composite sampling to be done)

22. Ice boxes with ice block Sampling Equipment/items - Microbiological and Biological 1 Bottom Sampler 13 Match box 2 Cotton Roll for swab 14 Narrow mouth Polypropylene sample bottle 3 Cotton tape for labelling 15 Permanent Marker 4 Dark & light Bottles 16 Plankton Net 5 Ekman Dredge 17 Rectified Spirit 6 Field Note book 18 Rope 7 Fine sieves large size for 19 Safety Glasses/Lab goggles collecting benthos 8 Gum boot 20 Soft Brushes 1 inch & ½ inch 499 Hand gloves 21 Sterilized microbiological glass bottle with ground glass cap 10 Hand shovel 22 Tweezers broad/fine 11 Ice box with ice block 23 Wide mouth glass sampling bottles 12 Large Container for Bulk sample 24 Wide mouth polypropylene for Toxicity testing sample sampling bottles Sampling Equipment/items - Soil, Sediment, Sludge

1. Antiseptic hand wash 9 Plastic tray for compositing the sample

2. Bucket 10 Polyethylene Bags

3. Coring devices/core catchers 11 Rubber Gloves

4. Disposable gloves 12 Sampling Auger & shovel

5. Distilled water 13 Stainless steel Hand Shovel

6. Measuring tape 14 Stainless steel large spatula/spoon

7. Permanent marker pen 15 Wash Bottle

8. pH strips/pH meter for field 16 Wide mouth Sampling Jars measurement of pH (Plastic/glass) Sampling Equipment/items- Industrial solid waste, Hazardous waste & Municipal Solid waste, Plastic waste 1 Antiseptic Hand wash 16 Rubber Gloves 2 Bucket 17 Sampling Auger & shovel 3 Coliwasa (Composite Liquid 18 Sampling Auger waste sampler) Glass & Plastic 4 Coring devices/core catchers 19 Sampling Bags (Polythene) 5 Disposable gloves 20 Sampling Trier 6 Distilled water 21 Sieves set 7 Double Protection Filter Chemical 22 Stainless steel Hand Shovel Gas Respirator 8 Field Note Book 23 Stainless steel large spatula/spoon 9 Grain sampler 24 Stainless steel Scoop/Trovel 10 Measuring tape 25 Tray for compositing the sample 11 Permanent Marker pen 26 Veihmeyer sampler 12 Polythene bags thickness Gauge 27 Wash Bottle

(Digital) 13 Pond Sampler 28 Waste pile sampler 14 PP/PE Plastic bottles 29 Weighted Bottle Sampler 15 PPE Kit including safety 30 Wide mouth Glass Bottles /Jars goggles/face mask Sampling Equipment/items- Ambient Air/Fugitive Emissions (Manual) 1 Absorbing solutions separate for 18 Laboratory safety goggles separate gases to be monitored 2 Air Flow meter 19 Low Volume Sampler (LVS) 3 Air pollution mask 20 Maximum Minimum Thermometer 4 Air Sampling Data sheet 21 Permanent Marker 5 Anemometer 6 Breathing apparatus 22 Personal Air Samplers 7 Dichotomous Sampler (PM2.5 23 Polyuretahane Foam (PUF) and PM10) Sampler 8 Disposable gloves 24 PP/PE Sampling bottles narrow mouth leak proof 9 Double Protection Filter Chemical 25 Pre weighed & coded EPM Gas Respirator 2000 Filter paper (Air Monitoring) 5010 Drager's Pump and Dragers 26 Pre weighed & coded Glass Detection tubes for specific air Fibre (GFA) Filter paper (Air pollutants monitoring) 11 Dust Fall Sampler 27 Rain Gauge 12 Envelops for filter paper storage 28 Respirable Dust Sampler (RDS) (*4 Nos) 13 Glass adsorption tubes 29 Spare carbon brushes 14 Glass Impingers 35 ml 30 Tedler bags 15 Handy Sampler with set of glass 31 Top loading orifice kit (For impingers (*2 Nos) calibration of HVS) 16 High Volume Sampler (HVS) 32 Weather Monitoring system 17 Laboratory Coat 33 Wind speed/wind direction monitor Sampling & Analytical Instrument/Equipment/items- Continuous Ambient Air Monitoring 1 Continuous Ambient Air 13 Continuous Ambient Air Monitoring System (Fixed) Monitoring System (Mobile) 2 Continuous PM Analyser 14 Continuous PM Analyser 10 2.5 3 Continuous PM & PM 15 Ambient Sulphur Dioxide 10 2.5 Monitoring Analyser (TEOM Analyser system) 4 Ambient Nitrogen Oxides (NO- 16 Ambient Carbon Monoxide & NO -NO ) Analyser Carbon dioxide Analyser 2 x 5 Ambient Ozone Analyser 17 Total Hydrocarbon Analyser 6 Ambient BTEX Analyser 18 Ambient Ammonia Analyser 7 Multipoint Gas Calibration 19 Zero Gas Generator system 8 19 inch Rack mounting system for 20 Air Sampling Probe Mast air analysers 9 Meteorological tower (All in one 21 Calibration Gas Cylinders (SO , 2 telescopic Mast) with sensors NO, CO, NH . Benzene and 3 comprising wind speed, wind Toluene (One each) with direction, ambient temp., Relative stainless steel Regulators humidity, Solar radiation, rainfall etc.

10 Synthetic Air Cylinder 22 Nitrogen Cylinder 11 Permeation tubes (SO , NO-NO - 23 Air Conditioners (Split type) 1.5 2 2 NOx,NH , BTX ton & 2.0 ton 3 12 Computer Station with Modem 24 UPS system 5 KVA & UPS system 15 KVA Sampling Equipment/items- Source Emissions 13 Isokinetic Stack Monitoring Kit (complete with Stack monitoring 1 Activated Charcoal tubes instrument panel with inclined cum vertical manometer, Cold Box, Vacuum pump, Glass assembly impingers 2 Asbestos gloves 14 Metal braided Flexible umbilical cord 15 Modified S type Stainless steel 3 Barometer (digital) Pitot tube (Standard length) with Assembly 4 Continuous emission monitoring 16 Monoblock type, rotary design equipment vacuum pump 5 Dry Gas Meter 17 Orsat Apparatus 6 Electricity Generator 18 Pre weighed and coded cellulose and fibre glass thimbles 7 Face Shield & helmet 19 Protective apron 8 Fire Extinguisher 20 Soap Bubble meter 5121 Source emission monitoring 9 First Aid box Impingers train set with spare impingers (100 ml & 225 ml capacity) 22 Stainless steel heated Sampling 10 Flue Gas Analyser Probes with thimble holders (short and long) 11 Gas Mask 23 Stop watch 12 Isokinetic Nozzles (Different sizes 24 Thermocouple probe nozzles set) Sampling & Analytical Equipment - Vehicular Emission 1 Carbon Dioxide Analyser 4 Multigas Analyser (petrol, CNG, LPG) 2 Diesel Exhaust Analyser 5 Opacity meter (Diesel) 3 Exhaust CO/HC Analyser with 6 Smoke Density meter Sampling Probe Sampling & Analytical Equipment- Ambient Noise/Source Noise 1 Automated Noise Monitoring 3 Digital Sound level (Noise) System Meters 2 Calibrator for Noise Meters 4 Integrating Sound level meter List of Environmental sample processing instruments / Equipment (alphabetically arranged) at Environmental Laboratory S.No. Name of Instruments/ Equipment Nos. S.No. Name of Instruments/ Equipment Nos.

Availab Availab le le Environmental Sample Processing Equipment 1 Accelerated Solvent Extraction 31 Laboratory Pestle mortar

(ASE) System 2 Aerosol Generator 32 Laboratory Ball Mill 3 Ammonia distillation assembly Laboratory Grinder 4 Analytical Balance (Digital) 33 Laminar Flow bench for Microbiological analysis 5 Aquarium for Toxicity bioassay 34 Magnetic Stirrer with or without test (*4 Nos) heating system (*2 Nos) 6 Arsenic Estimation assembly 35. Mechanical Shaker 7 Autoclave (*2 Nos) 36 Membrane Filtration assembly with vacuum pump 8 Bacteriological bottles 300 ml 37 Microbial culture refrigerator 9 Bacteriological Incubators- 38 Microwave Digester (Stainless steel) (*2 Nos) 10 Bio safety cabinets 39 MPN Bacteriological tubes with stand 11 BOD Incubators 40. Muffle Furnace ((*1 Nos)) 12 Centrifuge 41 Multi-parameter Kit for pH, Temperature, DO and Conductivity 13 COD Digestion heated Blocks 42 Petri Dishes 14 Cyanide Distillation Assembly 43 Phenol distillation assembly 15 Deep Freezer (Horizontal) 44 Plate counter (Manual/Automatic) 16 Deep Freezer (Vertical) 45 Precision Digital Balance (minimum detection

0.001mg/0.01 mg) 17 Digestion Chambers 46 Refrigerators Big Size 300 ltrs or more 5218 Digital Thermometer 47 Rotary Evaporator (Buchi type) with water recirculating chiller 19 Dispensers (Various capacities) 48 Separating funnel shaker 20 Filtration Assembly with vacuum 49 Separating Funnels for Liquid pump Liquid extraction 21 Fluoride Distillation Assembly 50 Soxhlet Apparatus 22 Glass Distillation assemblies 51 SPE/SPME Extraction system (Arsenic/ Fluoride/) 23 Glass Double Distillation 52 Spirit lamp/burner Assembly 24 Heating mantles 53 Thermometer 25 Hot Air Ovens (*2 Nos) 54 Thermometer (Dry & wet bulb) 26 Hot plates (small, Medium and 55 Toxicity characteristic leaching Large) (*2 Nos) procedure (TCLP) extractor 27 Hygrometer 56 Ultra sonic water bath 28 Imhoff Cone 57 Vacuum Pump with filtration assembly 29 Inoculation loop 58 Water Bath with temperature control (*2 Nos) 30 Kjeldhal Apparatus 53ANNEXURE VII Analytical Instruments at Environmental laboratories S. Name of Instrument No.

1. Atomic Absorption spectrometer (AAS)

2. Automatic Titration Assembly

3. Binocular Microscope

4. Binocular Stereo Zoom Microscope

5. Bomb Calorimeter

6. BTX Analyzer with BTX calibrator

7. Ca rbon, Hydrogen, Nitrogen and Sulphur (CHNS) Elemental Analyzer

8. Co lony counter

9. Co nductivity meter (*2 Nos)

10. Di gestion chamber(*01 Nos)

11. Di gital Burettes

12. Di gital Mercury Analyser

13. Di ssolved Oxygen Meter (Bench model)

14. ED XRF Analyser

15. Fla me Photometer (*1 Nos)

16. Fla sh Point Apparatus

17. Flo cculator ( Jar testing apparatus)

18. Fo urier-transform infrared Spectrometer (FTIR)

19. Ga s Chromatograph Mass Spectrometer with Head Space analyser

20. Ga s Chromatograph Mass Spectrometer with Purge & Trap

21. Ga s Chromatograph TOF-MS

22. Ga s Chromatograph with Electron capture detector and Flame photometric Detector GC- ECD/FPD

23. Ga s Chromatograph with Flame ionization detector GC-FID

24. Ga s Chromatograph with Mass Spectrometer (GC-MS)

25. Ga s Chromatograph with Mass Spectrometer (GC-MS-MS)

26. Gr aphite Furnace Atomic Absorption Spectrometer (GFAAS) with Vapour Generation Assembly

27. Hi gh Performance Liquid Chromatograph (HPLC)

28. Hi gh Resolution Mass Spectrometer (HRGC-HRMS)

29. Ind uctively Coupled Plasma (ICP) Spectrometer-OES

30. Ind uctively Coupled Plasma Mass (ICP-MS) Spectrometer

31. Ion Chromatograph Anion & Cations

32. Liq uid Chromatograph with Mass Spectrometer (LC-MS-MS)

33. Me thane and Non Methane (NMHC) Analyser

34. Mi croscope

35. Mi croscope Inverted

36. Mi croscope Binocular Research 38 Moisture Content Meter

37. CO (NDIR based) analyser(*1 Nos)

38. Ne phelometer (Turbidity Meter) 54S. Name of Instrument No.

39. No ise level Meter (*2 Nos)

40. Or ganic halogen (AOX/TOX) Analyser

41. pH -Meter with combined electrode

42. Sp ecific ion Analyser with ion selective electrodes

43. Sp ectrophotometer (Visible)

44. Ste reoscopic Microscope

45. TK N Analyser semi-automatic with aluminium block digester

46. TO C Analyser

47. To xic Gas Analyser

48. UV -Vis Spectrophotometer

49. W avelength Dispersive X-ray Fluorescence (WDXRF)

50. X Ray Fluorescence (XRF) Spectrometer (Portable)

51. Ze ro Head Space Extractor (ZHE) 55ANNEXURE VIII Laboratory Safety / Protection Equipment S. No Safety/Protection Equipment S. No Safety/Protection Equipment

1. Lab Coats with name plate 20. Fire blanket

2. Full body Aprons 21. Safety storage cabinet

3. Gloves heat protecting non absorbent 22. Chemical splash apparel gloves (Insulated)

4. Acid and Alkali proof gloves 23. Safely belts

5. Gloves (Latex examination gloves , 24. Rope (thick) Non sterile)

6. Gloves (Nitrile examination gloves , 25. Knee caps Non sterile)

7. Gloves (Nitrile examination gloves , 26. Helmets sterile)

8. Hard toed shoes ( for transportation 27. Fire proof jackets of cylinders)

9. Gum Boots 28. Shoe cover

10. Safety Goggles 29. Slippers

11. Tongs (big size) 30. Shoe rack

12. Tongs (small size) 31. Safety Harness

13. Hand Truck for transporting 32. Wadding suit cylinders

14. Sample trollies 33. Hand Protector grip

15. First Aid Boxes 34. Fire extinguisher

16. Gas Masks (Plastic) N-95 35. Solvent cabinets

17. Masks (particulate respire) 36. Chemical spill kits

18. Safety hand shower 37. Segregated Waste disposal containers for broken glass, chemical waste, plastic ware, paper. Electronic waste / batteries etc.

19. Stretcher 56ANNEXURE IX CPCB Inter-laboratory AQC Participation Fees Environmental Laboratories have to participate mandatorily in the Inter Laboratory Proficiency Testing program conducted by CPCB Annually as per the plan posted on CPCB official website.

Environmental Laboratories have to submit participation fees for participation in respective Inter- laboratory AQC Exercise. The fee shall be submitted only through online transfer (no other means of deposit shall be accepted).

The Inter Laboratory AQC Fee Structure for Environmental laboratories is given below:

Laboratories Inter laboratory AQC Participation Fee Private Laboratories Rs.30,000/= per year as per revised Gazette

Notification Laboratories of Govt./ Semi Rs.20,000/= per year as per revised Gazette Govt./ Public Sector Notification undertaking/ Autonomous 57ANNEXURE X TERMS & CONDITIONS FOR RECOGNITION OF LABORATORIES UNDER THE ENVIRONMENT (PROTECTION) ACT, 1986 The following terms and conditions shall be observed for recognition of laboratories under Section 12 (1) (b) of the Environment (Protection) Act, 1986.

1. The laboratory (Private/NGOs) shall be legally identifiable and registered with an appropriate statutory body i.e. local govt., State govt. or Central Govt.

2. The laboratory shall perform all the functions as mentioned in Rule 9 of the Environment (Protection) Rules, 1986.

3. It shall carry out the tests by using only validated/verified methods prescribed internationally/nationally are envisaged.

4. The test report shall be recorded in the standard format in triplicate. It shall be signed by the Government Analyst and be sent to the officer from whom the sample is received by the laboratory.

5. It shall carry out those tests, which are specified in the application and it shall not carry out any other test on the samples given.

6. The laboratory shall charge rates not exceeding those fixed by Central Pollution Control Board under Schedule of Analysis Charges as per the gazette Notification no. S.O./42(3)/87 dated 23rd February

2022.

7. The laboratory shall not charge rates higher than the rates they charge to any other Government or Public Sector organization.

8. The laboratory shall ensure that a Legal sample submitted to it for testing will only be tested by the authorised person and supervised by the `Government Analyst' by Central Government under provisions of the Environment (Protection) Act and as notified in the official gazette from time to time.

9. When a Government Analyst ceases to be in the services of the Laboratory, the Head of the Laboratory shall report this fact to the CPCB within fifteen days and simultaneously take steps for filling up this vacancy.

10. Any report signed by the Government Analyst may be used as evidence of facts in a court of law as per Section 14 of the Environment (Protection) Act, 1986. The laboratory shall provide all facilities to the `Government Analyst' for giving evidence in a court of law, if it becomes necessary.

11. It shall maintain complete secrecy in respect of the test results. These shall not be divulged to any person or authority other than the Officer empowered under Section 11 of the Act of the court having jurisdiction.

12. Laboratory shall remain open for all working days except weekly off, Central & State Govt. holidays.

Environmental laboratory of an educational institute/college will make arrangement of acceptance of samples and their analysis during any vacation exceeding more than 5 days i.e. summer/winter vacation etc.

13. It shall maintain proper records and registers and the calculations and test results in respect of tests conducted by them.

14. The laboratory and the Government Analysts employed by the laboratory shall participate in (Inter Laboratory- Analytical Quality Control Exercises) organized by the Central Government/ CPCB or an organization designated by it to test the capabilities of the recognized laboratories and analysts from time to time. The fee for participation in Inter Laboratory Analytical Quality Control exercise has to be paid by the participating laboratory to the designated organization.

5815. If feel necessary, Central Government will send dummy environmental samples to the laboratory to keep constant check over the laboratories of the results of the sample, which are to be analysed, free of cost by the laboratory and results will be provided to the Central Government.

16. If the laboratory is sent samples from an establishment with which it has got connections through ownership or other means which make it improper for the laboratory to carry out the tests with respect to that sample, it shall disclose the fact to the empowered officers or authority sending the sample and shall refuse the samples.

17. It shall be the responsibility of the laboratory to maintain properly the necessary infrastructure for conducting tests successfully.

18. In case the laboratory desires to make a mention of its recognition as environmental laboratory in its letter heads, printed material, signboards, etc., it shall specify the period of recognition and such mention of the recognition shall cease immediately after the expiry of recognition.

19. The laboratory shall comply with all the rules and regulations notified under the Environment

(Protection) Act, 1986.

20. The recognition shall become effective from and to the date stated in provisional certificate issued by Central Government.

21. CPCB shall have the right to revoke the recognition or de-recognize the laboratory at any time in public interest without assigning any reason, if it is deemed necessary by the Central CPCB.

22. The laboratory shall maintain complaint register (bounded and numbered)

23. The recognition accorded to Government Analysts in an environmental laboratory ceases along with the de-recognition of that environmental laboratory.

24. In case there is any change in Location or name of the laboratory for which it has been granted recognition earlier the laboratory will inform Central Govt./CPCB regarding the change as well as apply afresh for recognition.

25. In case of takeover of a recognized private laboratory, its ownership changes; occurrence of such changes must be communicated to the recognition body Central Government/CPCB within one month. Through, an appropriate mechanism that the laboratory continues to comply with the criteria against which recognition was originally granted will be verified.

26. After recognition, laboratory can be re-inspected at any time for its periodic surveillance/assessment/performance.

The aforesaid terms and conditions are acceptable to us.

Dated:

Signature ____________________________ (Head of Laboratory) Full Name _____________________________ (in capital letters) Address _____________________________ 59ANNEXURE XI TERMS AND CONDITIONS ACCEPTANCE BY PROPOSED GOVERNMENT ANALYSTS UNDER THE ENVIRONMENT (PROTECTION) ACT, 1986

1. A person working as analyst in a laboratory recognized as Environmental Laboratory under the Environment (Protection) Act, 1986 may be recognized as a Government Analyst if he has the qualifications and experience as prescribed in the Rule 10 of the Environment (Protection) Rules, 1986.

2. The recognition of a Government Analyst is with respect to the laboratory where he is employed at the time of recognition and which is recognized as an environmental laboratory under Section 12 of the Environment (Protection) Act, 1986. If he ceases to be in the services of the said laboratory, his recognition ceases forthwith from the date of termination of his service with the laboratory.

3. The recognition accorded to the Government Analyst in an Environmental Laboratory ceases along with the expiry of recognition/ revoking of recognition/de-recognition of that environmental laboratory.

4. The Government Analyst shall participate in any `Round Robin Test' organized by the Central Government or an organization designated by it for testing his capabilities for conducting various environmental tests.

5. It shall be the responsibility of the Government Analyst to see that the instruments necessary for conducting various environmental tests are properly maintained and calibrated.

6. The test report by the Government Analyst submitted to the appropriate authority shall bear the logo/ seal of the laboratory/ Organization.

7. The Government Analyst shall appear in a court of law for giving evidence, If required by the appropriate court / Central Govt.

8. The Government Analyst shall maintain complete secrecy in respect of test results.

9. The Government Analyst shall comply with the rules and regulations notified under the Environment

(Protection) Act, 1986.

11. The Central Government/ CPCB shall have the right to derecognize the Government Analyst any time in public interest without assigning any reason.

The undersigned accept the above terms and conditions for consideration of recognition as Government Analyst as per Section 13 of Environment (Protection) Rules, 1986 S. No Name of proposed Govt Analyst Designation Qualifications Signature

1.

2.

3.

Countersignature Head of Laboratory SEAL of LABORATORY 60ANNEXURE XII BIO-DATA PROFORMA FOR CONSIDERATION OF RECOGNITION AS GOVT. ANALYST UNDER THE ENVIRONMENT (PROTECTION) ACT, 1986

1. Name in full (In block letters) : _____________________________________

2. Father’s name : _____________________________________

3. Date of birth : _____________________________________

4. Nationality : _____________________________________

5. Permanent Address : _____________________________________ _____________________________________

6. Tel No. ________________ Fax _______________ E-Mail ______________

7. Educational qualification (Give detail in chronological order from graduation & onward):

S. Name of Board/ University/ Year of Exam passed Subjects taken Division No. Institution passing

1.

2.

8. Professional Training taken during last 3 years:

S. Period of training Organization Subject of the training No. From To

9. Previous and current employment records:

Type of organization Period of service Reason S. Name & address Govt./Semi-Govt./ Salary Job Post held for No. of employer Public Sector/ From To drawn nature leaving Private/NGO

Note: Please attach separate sheet, if space is inadequate.

10. Experience in Analysis of Environmental Samples ________ years.

11. Please provide details if earlier appointed / recognized as Govt. Analyst under The Environment

(Protection) Act, 1986:

12. Declaration:

I declare that the foregoing information is correct and complete to the best of my knowledge and belief and nothing has been concealed / distorted. Documents in support of my educational qualification and experience will be provided, if required so.

Place: (Signature of candidate)

Date:

Signature of the Head of the Laboratory SEAL OF THE LABORATORY 61ANNEXURE XIII Draft Gazette Notification TO BE PUBLISHED IN THE GAZETTE OF INDIA EXTRAORDINARY PART ………. SECTION ………SUB-SECTION……..

CENTRAL POLLUTION CONTROL BOARD MINISTRY OF ENVIRONMENT & FORESTS

NOTIFICATION New Delhi, Dated ………………..

No. _______________In exercise of the powers conferred by clause (b) of Sub-section 1 of Section 12 and Section 13 read with clause 1 of the Notification No. 145(E), dated 21st February, 1991, issued under Section 23 of the Environment (Protection) Act, 1986 (29 of 1986), the Central Pollution Control Board, PariveshBhawan, East Arjun Nagar, Delhi - 110032 hereby recognizes, (a) the laboratories specified in column (2) of the Table below as ‘Environmental Laboratories’ to carry out the functions entrusted to the environmental laboratories under the said Act, and the rules made thereunder, and (b) specified persons in column (3) of the Table as the Government Analysts for the purposes of analysis of samples of air, water, soil or other substances sent for analysis, specified for respective groups of parameters mentioned in Column (4).

After Sr. No.______ of the Notification No. ____________, dated ___________, and the entries relating thereto, the following Serial Nos. and entries shall be added namely:- S. Name of Name of the Govt. Recognized for Validity No. Laboratory analysts parameter groups

1. 2. 3. 4. 5.

The Environmental Laboratories so mentioned shall remain valid as per column (5) from the date of Gazette notification. However, Government Analysts so mentioned at column (3) shall be appointed for a validity period mentioned at column (5) or up to the superannuation from the service of the Organization, whichever is earlier.

Authorized Signatory CPCB / MoEF

Foot Note:

The principal notification was published in the Gazette of India vide SO NO. 728(E), dated 21.7.1987 and

subsequently amended vide:-

(I)SO 838(E), dated 23.9.87 (2) SO 989(E) dated 17.11.87 (3) SO 489(E) dated 17.5.88 (4) SO 156 (E) dated

24.2.89 (5) SO 846(E) dated 24.10.89 (6) SO 375(E) dated 26.4.1990 (7) SO 803(E) dated 23.9.92 (8) SO 97(E) dated 5.12.1994 (9) SO 418(E) dated 31.3.1996 (10) SO 889 (E) dated 31.8.1996 (11) SO 452 (E) dated 31.5.97

(12) SO 631 (E) dated 31.5.1998 (13) SO (E) 336 dated 1.1.1999(14) SO.44(E), dated 15.1.2001, (15) S.O.No.490(E), dated 1.6.2001, (16) S.O. No. 532(E), dated 1.1.2002, (17) S.O. No.1168(E), dated 1.6.2002, (18) S.O.No.888(E), 1.6.2003, (19) No.Legal/42(3)/87/, dated 1.6.2004, (20) Legal/42(3)/87, dated 1.3.2005, (21) Legal42(3)/87, dated 1.9.2005, (22) Legal 42(3)/87, dated 15.12.2005 and (23) Legal 42(3)/87, dated 1.5.2006.

62Addendum I 636465Addendum II 66676869Addendum III 7071Addendum IV GLOSSARY OF TERMS - LABORATORY QUALITY ASSURANCE (QA) AND QUALITY CONTROL (QC) SYSTEM

1. Accreditation Formal recognition of the competence of a body or an organization for a well-defined purpose. It is the procedure by which a laboratory is assessed to perform a specific range of test or measurements

2. Accuracy The closeness of agreement between the "true" value and the measured value. The smaller the systematic error of the analysis is, the more accurate is the analytical procedure. It is assessed by means of reference samples and percent recoveries

3. Audit Sample Prepared reference sample inserted into the sample processing procedure as close to the beginning as possible.

4. Background Sample A sample taken from a location on or proximate to the site of interest and used to document baseline or historical information

5. Bias Systematic error, consistent deviation of measured values from the true value.

6. Calibration In chemical measurement, Calibration refers to the process by which the response of a measurement system is related to the concentration or the amount analyte of interest

7. Calibration Laboratory Laboratory that performs calibration.

8. Calibration Method Defined technical procedure for performing a calibration.

9. Calibration Standards A series of known standard solutions used by the analyst for calibration of instrument (i.e. preparation of the analytical curve).

10. Certified Reference A certified reference material is a material or substance whose Material (CRM) property or properties can be defined so exactly that it may be used for the calibration of measuring instruments, the check of results obtained from measuring, testing and analytical processes, and for the characterization of substance properties.

11. Chain of Custody (COC) Documentation of the history of the sample. The components of chain of custody are sample seals; log book, record and sample analysis request sheet and the procedures used for estimation.

12. Confidence Set of possible values within which the true value will lie with a Interval specified level of probability.

13. Confidence Limit One of the boundary values defining the confidence interval

14. Contamination Something inadvertently added to the sample during the sampling or analytical process.

15. Control Type of sample against which the results of a procedure are judged.

16. Conventional true value Value attributed to a particular quantity and accepted, sometimes by convention, as having an uncertainty appropriate for a given purpose

17. Data Quality Objectives Statements on the level of uncertainty that a decision-maker is willing

(DQOs) to accept in the results derived from environmental data.

18. An environmental sample or field sample is a representative sample of Environmental sample any material (aqueous, no aqueous or multimedia) collected from any source for which determination of composition of contamination is requested or required.

19. Error Difference between a measured value and the true value.

20. Good Laboratory Good laboratory Practice(GLP) is concerned with the organizational Practice(GLP) process and the conditions under which laboratory studies are planned, monitored, recorded and reported

21. Which makes it necessary to begin a new analysis (Ex. using a wrong Gross error reagent, taking a wrong pipette, measuring at a wrong wavelength. instrument breakdown, heavily contaminated glassware etc.). These errors should easily be recognized.

22. Instrumentation The concentration equivalent to a signal due to the analyte which is Detection Limit equal to three times the standard deviation of a series of 7 replicate measurements of a reagent blank’s signal at the same wave length

23. Inter Laboratory (Reproducibility) Variation associated with two or more laboratories or Precision organizations using the same measurement method

7224. Inter Laboratory A series of measurements of one or more quantities performed Test independently by a number of laboratories on samples of a given material (other terms: Round robin test, Collaborative trial, Collaborative reference program, Collaborative analytical study, ring test).

25. Compounds whose presence obscures the measurement of the Interferences analyte of interest by the introduction of an unrelated analytical signal where the analyte is measured.

26. Internal Quality Control Internal quality control encompasses all measures, which are planned, ordered and executed by a laboratory itself

27. Intra-laboratory (Repeatability) Variation associated with a single laboratory or Precision organization.

28. Limit of detection (LOD) The LOD of an individual analytical procedure is the lowest analytical amount of an analyte in a sample, which can be detected but not necessarily quantified as an exact value. For many purposes, the LOD is arbitrarily taken to be 3sb or 3 x the standard deviation of the blank value or of background

29. Limit of Determination The lower level where measurements become arbitrarily meaningful and is defined arbitrarily as LOQ-10sb (10 x the standard deviation of the blank value or of background) At this concentration, the relative confidence in the measured value is ± 30% at the 95% confidence level.

30. Limit of Quantitation The constituent concentration that produces a signal sufficiently

(LOQ) greater than it can be detected within specified limits by good laboratories during routine operating conditions. Typically, it is the concentration that produces a signal time 10s above the reagent water blank signal.

31. Matrix The matrix of a material is the totality of all parts of a material and their chemical and physical properties including mutual influences

32. Matrix /Spike Duplicate In matrix/spike duplicate analysis, predetermined quantities of stock Analysis solutions of certain analytes are added to a sample matrix prior to sample extraction/digestion and analysis. Samples are split into duplicates, spiked and analyzed. Percent recoveries are calculated for each of the analytes detected. The relative percent difference between the samples is calculated and used to assess analytical precision. The concentration of the spike should be at the regulatory standard level or the estimated or actual method quantification limit. When the concentration of the analyte in the sample is greater than 0.1% no spike of the analyte is necessary.

33. Method Detection Limit The minimum concentration of a substance that can be measured and

(MDL) reported with 99 % confidence that the analyte concentration is greater than zero. The MDL is determined from analysis of a sample in given matrix containing analyte which has processed through the pre- operative procedure

34. Method Quantification The Method Quantification Limit is the minimum concentration of a Limit (MQL) substance that can be measured and reported.

35. Optimum Concentration A range defined by limits expressed in concentration, below which Range scale must be used and above which curve correction should be considered. This range varies with the sensitivity of the instrument and the operating conditions employed.

36. Outlier data The data, which are suspected to be extremely low or high from the expected value or mean.

37. Precision The closeness of agreement between the results obtained by applying the experimental procedure several times under prescribed conditions. (The smaller the random part of the experimental errors, which affect the results, the more precise is the procedure). Within run and between day precisions have to be considered. (See repeatability and reproducibility)

38. Proficiency Testing Determination of the laboratory calibration or testing performance by means of inter-laboratory comparisons.

39. Protocol Thorough written description of the detailed steps and procedures involved in the collection of samples.

40. Quality Assessment Procedure for determining the quality of laboratory measurements by use of data from internal and external quality control measures.

7341. Quality assurance All those planned and systematic actions necessary to provide adequate confidence that a product or a service will satisfy given requirements for quality

42. Quality Assurance An orderly assemblage of management policies, objectives, principles and Programme Plan (QAPP) general procedures by which an organization involved in environmental data generation activities outlines how to produce data of known quality.

43. Quality Assurance An orderly assemblage of detailed procedures designed to produce Project Plan (QAPP) data of sufficient quality to meet the DQOs for a specific data collection activity.

44. Quality Characteristic The characteristics and characteristic values (or expressions) of something (e.g. a method, a piece of equipment, a measurement result etc.) in relation to their suitability to fulfill set requirements.

45. Quality Control Set of measures within a sample analysis methodology to assure that the process is in control.

46. Quality Control Charts A Quality control chart is a sequential plot of some quality characteristic. It may be a day-by –day measurement of any interest of analyte (e.g. COD or BOD or Nitrate). The Chart consists of central line and two pairs of limit lines, the Upper and Lower Warning Limits (UWL, LWL) and Upper and Lower Control Limits (UCL., LCL) Example :

Shewart’s Quality Control Chart

47. Quality Manual A document stating the quality policy, quality system and quality practices of an organization.

48. Quality System The organizational structure, responsibilities, procedures, processes and resources for implementing quality management.

49. Random Errors Random errors are indicated by the scatter of the results of repeated measurements on the aliquot of same sample about the mean value. The sign and magnitude of the error of any particular result varies at random and cannot be known exactly. Random errors arise from uncontrolled variations in the conditions of the analytical system (factors like analyst, equipment, instrument, method, quality of glassware and chemicals, reagents etc.,) during different analysis.

50. Range Spread of values calculated by subtracting the lowest value from the highest value

51. Reagent Blank A reagent blank is an aliquot of analyte –free water or solvent analysed with the analytical batch.

52. Reference material A material or substance one or more of whose property value are sufficiently homogeneous and well established to be used for the calibration of an apparatus, the assessment of a measurement method, or for assigning value to materials

53. Reference Standard A standard, generally of the highest meteorological quality available at a given location, from which measurements made at that location, is derived.

54. Relative Standard Estimate of the average error in the measurement due to unassignable Deviation (RSD) causes and usually expressed as a percentage of the average sample concentration.

55. Repeatability The closeness of agreement between successive results obtained with the same method on identical test material under the same condition. (Same operator, same apparatus, same laboratory and short intervals of time) can also be interpretable as within – run precision.

56. Replicate Sample A replicate sample is a sample prepared by dividing a sample into two or more separate aliquots. Duplicate sample is considered to be two replicates.

57. Replicate Sample A replicate sample is a sample prepared by dividing a sample into two or more separate aliquots. Duplicate sample is considered to be two replicates.

58. Reproducibility The closeness of agreement between individual results obtained with the same method and on identical material but under different test conditions (different operator, different time’s etc.) Can also be interpreted as "Between-run precision".

59. Sampling Attempt to choose and extract a representative portion of a physical system from its surroundings.

7460. Sensitivity Sensitivity describes the ability of an experimental method to differentiate between related values (e.g. concentrations). It indicates to which degree value changes depending upon the signal of the measuring system and can be quantified using the slope of the calibration curve.

61. Standard Curve A standard curve is a curve, which plots concentrations of known analyte standard versus the instrument response to the analyte.

62. Standard Deviation Square root of the variance (statistical analysis).

63. Standard Methods A standard method is an acknowledged analytical method according to an international or national standard or guidelines or to a given legal statue.

64. Standard Operating Standard Operating procedure (SOP) means written procedure, which Procedure (SOP) describes how those routine laboratory operations, are to be performed.

65. Stratified Random Sampling technique in which estimates of strata means are combined to Sampling yield estimates of the population mean.

66. System Blank (Instrument Blank): Measure of the instrument background or baseline response in the absence of a sample.

67. Systematic Error Systematic errors are indicated by a unidirectional tendency of results, which could be greater or smaller than the true value. When systematic error is present, the result are said to be biased (bias systematic error) Systematic errors in analytical results may occur when method used is not specific for the analyte; due to presence of some interfering substances; improper performing of the analysis, instability of samples between sample collection and analysis.

68. Test A technical operation that consists of the determination of one or more characteristics or performance of a given product, material equipment organism, physical phenomenon, process or service according to a specified procedure.

69. Test Method Defined technical procedure for performing a test

70. Traceability The property of a result of a measurement whereby it can be related to appropriate standards, generally international or national standards, through an unbroken chain of comparisons.

71. True value Real amount or concentration of an analyte in a certain sample. It is an ideal value, which could be arrived at only if all causes of measurement error was eliminated. The amount or concentration given for an analyte in a certified reference material is a good substitute for the true value.

72. Trueness The closeness of agreement between the average value obtained from a large series of test results and an accepted reference value

73. Uncertainty of Parameter, associated with the result of a measurement, that measurement characterizes the dispersion of the values that could reasonably be attributed to the measured

74. Validation Validation is the total testing procedure if an analytical method is free of random and systematic error, not only within calibration but also and especially frees of interference when analyzing real samples.

75. Variance Measure of the variability in a population / set of analytical data. It is the square root of the standard deviation 75